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首页> 外文期刊>Acta biomaterialia >Stereolithography of spatially controlled multi-material bioactive poly(ethylene glycol) scaffolds.
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Stereolithography of spatially controlled multi-material bioactive poly(ethylene glycol) scaffolds.

机译：空间控制的多材料生物活性聚（乙二醇）支架的立体光刻。

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Challenges remain in tissue engineering to control the spatial, mechanical, temporal and biochemical architectures of scaffolds. Unique capabilities of stereolithography (SL) for fabricating multi-material spatially controlled bioactive scaffolds were explored in this work. To accomplish multi-material builds, a mini-vat setup was designed allowing for self-aligning X-Y registration during fabrication. The mini-vat setup allowed the part to be easily removed and rinsed, and different photocrosslinkable solutions to be easily removed and added to the vat. Two photocrosslinkable hydrogel biopolymers, poly(ethylene glycol) dimethacrylate (PEG-dma, MW 1000) and poly(ethylene glycol) diacrylate (PEG-da, MW 3400), were used as the primary scaffold materials. Multi-material scaffolds were fabricated by including controlled concentrations of fluorescently labeled dextran, fluorescently labeled bioactive PEG or bioactive PEG in different regions of the scaffold. The presence of the fluorescent component in specific regions of the scaffold was analyzed with fluorescent microscopy, while human dermal fibroblast cells were seeded on top of the fabricated scaffolds with selective bioactivity and phase contrast microscopy images were used to show specific localization of cells in the regions patterned with bioactive PEG. Multi-material spatial control was successfully demonstrated in features down to 500 microm. In addition, the equilibrium swelling behavior of the two biopolymers after SL fabrication was determined and used to design constructs with the specified dimensions at the swollen state. The use of multi-material SL and the relative ease of conjugating different bioactive ligands or growth factors to PEG allows for the fabrication of tailored three-dimensional constructs with specified spatially controlled bioactivity.
机译：在组织工程中控制支架的空间，机械，时间和生化结构方面仍然存在挑战。在这项工作中探索了立体光刻（SL）用于制造多材料空间控制的生物活性支架的独特功能。为了完成多种材料的构建，设计了一个微型容器设置，允许在制造过程中自动对齐X-Y对齐。微型容器设置使零件易于拆卸和冲洗，而不同的可光交联溶液也易于拆卸并添加到桶中。两种可光交联的水凝胶生物聚合物，聚（乙二醇）二甲基丙烯酸酯（PEG-dma，MW 1000）和聚（乙二醇）二丙烯酸酯（PEG-da，MW 3400），被用作主要的支架材料。通过在支架的不同区域中包括受控浓度的荧光标记的葡聚糖，荧光标记的生物活性PEG或生物活性PEG，来制造多材料支架。用荧光显微镜分析支架特定区域中荧光成分的存在，同时将人真皮成纤维细胞以选择性生物活性播种在所制造支架的顶部，并使用相差显微镜图像显示区域中细胞的特定定位具有生物活性PEG的图案。在低至500微米的特征中成功展示了多材料空间控制。此外，确定了SL制造后两种生物聚合物的平衡溶胀行为，并用于设计具有特定尺寸的溶胀状态的构建体。多种材料SL的使用以及将不同的生物活性配体或生长因子与PEG缀合的相对容易性，使得可以制造具有特定空间控制的生物活性的量身定制的三维构建体。

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