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首页> 外文期刊>Acta biomaterialia >Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration.
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Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration.

机译：通过化学合成制备的生物矿化的多孔复合支架，用于骨骼组织再生。

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摘要

Scaffold design is a key factor in the clinical success of bone tissue engineering grafts. To date, no existing single biomaterial used in bone repair and regeneration fulfils all the requirements for an ideal bone graft. In this study hydroxyapatite/polycaprolactone (HA/PCL) composite scaffolds were prepared by a wet chemical method at room temperature. The physico-chemical properties of the composite materials were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, while scaffold morphology was investigated by scanning electron microscopy (SEM) with energy-dispersive spectroscopy to validate the process used for synthesis. Finally, the response of bone marrow-derived human mesenchymal stem cells (hMSCs) in terms of cell proliferation and differentiation to the osteoblastic phenotype was evaluated using the Alamar blue assay, SEM and alkaline phosphatase activity. Microstructural analysis indicated that the HA particles were distributed homogeneously within the PCL matrix. The biological results revealed that the HA/PCL composite scaffolds are suitable for the proliferation and differentiation of MSCs in vitro, supporting osteogenesis after 15 days. All the results indicate that these scaffolds meet the requirements of materials for bone tissue engineering and could be used for many clinical applications in orthopaedic and maxillofacial surgery.
机译：支架设计是骨组织工程移植物临床成功的关键因素。迄今为止，尚无用于骨骼修复和再生的现有单一生物材料能够满足理想骨移植的所有要求。在这项研究中，羟磷灰石/聚己内酯（HA / PCL）复合支架是通过湿化学方法在室温下制备的。通过X射线衍射，傅里叶变换红外光谱和X射线光电子能谱对复合材料的理化性质进行了表征，同时通过扫描电子显微镜（SEM）和能量色散谱法研究了支架的形态，以验证所使用的过程用于合成。最后，使用Alamar蓝分析，SEM和碱性磷酸酶活性评估了骨髓来源的人间充质干细胞（hMSCs）在细胞增殖和分化方面对成骨细胞表型的反应。微观结构分析表明，HA颗粒在PCL基质内均匀分布。生物学结果表明，HA / PCL复合支架适用于MSC的体外增殖和分化，支持15天后的成骨作用。所有结果表明，这些支架符合骨组织工程材料的要求，可用于整形外科和颌面外科的许多临床应用。

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