首页> 外文期刊>Acta biomaterialia >Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique.
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Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique.

机译:使用Technovit 9100新的嵌入技术在硬组织与植入物界面处进行组织学和研究。

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摘要

Calcified tissues, like bones and teeth, are among the most challenging tissues for histological research. However, especially with respect to dental or orthopaedic research, powerful histological techniques are necessary to study pathological conditions or traumatic injuries, and to investigate the molecular and cellular mechanisms of regeneration processes and functional recovery. The situation is even more complicated in orthopaedic research because here metallic implants or other devices made of various materials are often present, and the hard tissue-implant interface is of crucial interest in both biocompatibility and functional recovery research. After the cutting-grinding technique, embedding in technical resins is the most promising approach. Here we describe an optimized and standardized embedding and cutting technique using Technovit 9100 New. Using this technique, we are able to perform enzyme histochemistry, immunohistochemistry, a great variety of classical histological stains and even in situ hybridization.
机译:钙化组织(如骨骼和牙齿)是组织学研究中最具挑战性的组织之一。但是,特别是在牙科或整形外科研究方面,强大的组织学技术对于研究病理状况或创伤性损伤以及研究再生过程和功能恢复的分子和细胞机制是必不可少的。在整形外科研究中,情况甚至更加复杂,因为这里经常会出现金属植入物或其他由各种材料制成的装置,而硬组织与植入物的界面在生物相容性和功能恢复研究中都至关重要。在切割研磨技术之后,将树脂嵌入技术树脂是最有前途的方法。在这里,我们描述了使用Technovit 9100 New优化和标准化的嵌入和切割技术。使用这种技术,我们能够进行酶组织化学,免疫组织化学,多种经典的组织学染色甚至原位杂交。

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