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首页> 外文期刊>Acta biomaterialia >Biotinylation of silicon-doped hydroxyapatite: a new approach to protein fixation for bone tissue regeneration.
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Biotinylation of silicon-doped hydroxyapatite: a new approach to protein fixation for bone tissue regeneration.

机译：硅掺杂的羟基磷灰石的生物素化：一种用于骨组织再生的蛋白质固定新方法。

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Silicon-doped hydroxyapatite has been functionalized with biotin molecules as a new methodology for the attachment of proteins, peptides or growth factors through the formation of avidin-biotin complex in this material. Bioceramic biotinylation has been performed by esterification reaction between the OH groups of hydroxyapatite and COOH groups of biotin molecules. Several parameters of the biotinylation, such as the addition of N,N'-dicyclohexylcarbodiimide (DCC), the biotin/bioceramic molar ratio and the activation time, have been studied in order to improve both the amount of anchored biotin on the bioceramic surface and its bond strength. The grafting of biotin on a silicon-doped hydroxyapatite surface was determined using (13)C nuclear magnetic resonance, Fourier transform infrared spectroscopy and elemental analyses. The results show that the addition of DCC significantly increases both the amount of biotin grafted and the bond strength, because the major part is through covalent bonding. Lixiviation studies in simulated body fluid (SBF) at 37 degrees C have confirmed such results, showing that the retention grade after 7 days in SBF was of ca. 63%. Fluorescein isothiocyanate-avidin complexation has been performed on three-dimensional (3-D) scaffolds prepared by a rapid-prototyping technique. Confocal microscopy studies show a homogeneous distribution with a higher incorporation rate of the protein over the entire external surface of the biotinylated 3-D scaffold.
机译：硅掺杂的羟基磷灰石已通过生物素分子功能化，成为通过在这种材料中形成亲和素-生物素复合物而附着蛋白质，肽或生长因子的新方法。已经通过羟基磷灰石的OH基团与生物素分子的COOH基团之间的酯化反应来进行生物陶瓷生物素化。研究了生物素化的几个参数，例如添加N，N'-二环己基碳二亚胺（DCC），生物素/生物陶瓷的摩尔比和活化时间，以同时改善生物陶瓷表面和表面上锚定的生物素的量。它的粘结强度。使用（13）C核磁共振，傅立叶变换红外光谱和元素分析确定了生物素在硅掺杂羟基磷灰石表面上的接枝。结果表明，DCC的添加显着增加了生物素接枝量和结合强度，因为主要部分是通过共价结合。在37摄氏度的模拟体液（SBF）中进行浸脂研究已证实了此类结果，表明在SBF中7天后的保留等级约为。 63％。荧光素异硫氰酸酯-亲和素络合物已在通过快速原型技术制备的三维（3-D）支架上进行。共聚焦显微镜研究表明，在生物素化的3-D支架的整个外表面上，蛋白质的掺入率较高，分布均匀。

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