• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Nanostructuring of PEG-fibrinogen polymeric scaffolds.
【24h】

Nanostructuring of PEG-fibrinogen polymeric scaffolds.

机译：PEG-纤维蛋白原聚合物支架的纳米结构。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies have shown that nanostructuring of scaffolds for tissue engineering has a major impact on their interactions with cells. The current investigation focuses on nanostructuring of a biocompatible, biosynthetic polymeric hydrogel scaffold made from crosslinked poly(ethylene glycol)-fibrinogen conjugates. Nanostructuring was achieved by the addition of the block copolymer Pluronic F127, which self-assembles into nanometric micelles at certain concentrations and temperatures. Cryo-transmission electron microscopy experiments detected F127 micelles, both embedded within PEGylated fibrinogen hydrogels and in solution. The density of the F127 micelles, as well as their ordering, increased with increasing block copolymer concentration. The mechanical properties of the nanostructured hydrogels were investigated using stress-sweep rheological testing. These tests revealed a correlation between the block copolymer concentration and the storage modulus of the composite hydrogels. In vitro cellular assays confirmed that the increased modulus of the hydrogels did not limit the ability of the cells to form extensions and become spindled within the three-dimensional (3-D) hydrogel culture environment. Thus, altering the nanostructure of the hydrogel may be used as a strategy to control cellular behavior in 3-D through changes in mechanical properties of the environment.
机译：最近的研究表明，用于组织工程的支架的纳米结构对其与细胞的相互作用具有重大影响。当前的研究集中在由交联的聚（乙二醇）-纤维蛋白原结合物制成的生物相容性，生物合成的聚合物水凝胶支架的纳米结构上。通过添加嵌段共聚物Pluronic F127实现纳米结构，该共聚物在一定浓度和温度下会自组装成纳米胶束。低温透射电子显微镜实验检测到F127胶束，既嵌入PEG化纤维蛋白原水凝胶，又嵌入溶液。 F127胶束的密度及其有序性随嵌段共聚物浓度的增加而增加。纳米结构水凝胶的力学性能使用应力扫描流变测试进行了研究。这些测试揭示了嵌段共聚物浓度与复合水凝胶的储能模量之间的相关性。体外细胞试验证实，增加的水凝胶模量并不限制细胞形成延伸并纺成三维（3-D）水凝胶培养环境的能力。因此，改变水凝胶的纳米结构可以用作通过改变环境机械性能来控制3-D细胞行为的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号