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首页> 外文期刊>Acta biomaterialia >Engineered dermal equivalent tissue in vitro by assembly of microtissue precursors.
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Engineered dermal equivalent tissue in vitro by assembly of microtissue precursors.

机译：通过微组织前体的组装在体外工程化真皮等效组织。

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Tissue-engineered constructs can be fabricated by the assembly of smaller building blocks in order to mimic much of the native biology that is often made from repeating functional units. Our aim was to realize a three-dimensional (3-D) tissue-like construct in vitro by inducing the assembly of functional micrometric tissue precursors (microTPs). MicroTPs were obtained by dynamic cell seeding of bovine fibroblasts on porous gelatine microcarriers using a spinner flask bioreactor. During the dynamic seeding, cells adhered, proliferated and synthesized a thin layer of extracellular matrix (ECM) in and around the macroporous beads, generating the microTPs. The analysis showed that the ECM produced was rich in type I collagen. The cells and ECM layer around the microTPs allowed their biological sintering via cell-cell and cell-matrix interaction after only a few days of dynamic seeding. The assembling ability of microTPs was exploited by placing them in a maturation chamber. After 1 week of culture disc-shaped constructs (1cm in diameter, 1mm in thickness) of completely assembled microTPs were collected. The biohybrid obtained presented both a homogeneous and compact aspect. Moreover, histological and immunohistochemical analyses revealed an abundant ECM, rich in type I collagen, interconnecting the microTPs. The results obtained in this survey pave the way to realizing a 3-D dermal tissue equivalent by means of a bottom-up tissue engineering approach.
机译：组织工程化的构建体可以通过组装较小的构建块来制造，以模仿通常由重复的功能单元制成的许多天然生物学。我们的目的是通过诱导功能性测微组织前体（microTPs）的组装，在体外实现三维（3-D）类组织构造。通过使用旋转瓶生物反应器将牛成纤维细胞动态接种在多孔明胶微载体上获得MicroTP。在动态接种过程中，细胞粘附，增殖并在大孔珠粒内和周围合成了一层薄薄的细胞外基质（ECM），从而产生了microTP。分析表明产生的ECM富含I型胶原。动态接种仅几天后，microTP周围的细胞和ECM层就可以通过细胞-细胞和细胞-基质的相互作用进行生物烧结。通过将microTP放置在成熟室中来发挥其组装能力。培养1周后，收集完全组装的microTP的碟形培养物（直径1厘米，厚度1毫米）。所获得的生物杂交体呈现均质和致密的方面。此外，组织学和免疫组织化学分析显示，ECM丰富，富含I型胶原，将microTP连接在一起。在这项调查中获得的结果为通过自下而上的组织工程方法实现3-D真皮组织等效物铺平了道路。

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