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首页> 外文期刊>Acta biomaterialia >Thin film composites of nanocrystalline ZrO(2) and diamond-like carbon: Synthesis, structural properties and bone cell proliferation.
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Thin film composites of nanocrystalline ZrO(2) and diamond-like carbon: Synthesis, structural properties and bone cell proliferation.

机译：ZrO（2）和类金刚石碳纳米晶的薄膜复合材料：合成，结构性质和骨细胞增殖。

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We report on the synthesis of thin composites of diamond-like carbon (DLC) and nanocrystalline ZrO(2) deposited using pulsed direct current plasma-enhanced chemical vapor deposition at low temperatures (<120 degrees C). Films containing up to 21at.% Zr were prepared (hydrogen was not included in the calculation) and their structural and surface properties were determined using a number of spectroscopic methods and contact angle measurements. Bone cell adhesion to the films was studied using a 3 day cell culture with osteoblasts. These nanocomposites (DLC-ZrO(2)) consist of tetragonal ZrO(2) nanocrystals with an average size of 2-5 nm embedded in an amorphous matrix consisting predominantly of DLC. The surface water contact angle of the films increased from approximately 60 degrees to 80 degrees as the Zr content increased from 0 to 21at.%. The cell culture study revealed that although the cell counts were not significantly different, the morphology of the osteoblasts growing on the DLC-ZrO(2) nanocomposites was markedly different from that of cells growing on DLC alone. Cells growing on the DLC-ZrO(2) surfaces were less spread out and had a smaller cell area in comparison with those growing on DLC surfaces. In some areas on the DLC-ZrO(2) surfaces, large numbers of cells appeared to coalesce. It is postulated that the difference in cell morphology between osteoblasts on DLC-ZrO(2) surfaces and DLC surfaces is related to the presence of very small tetragonal nanocrystals of ZrO(2) in the composite film.
机译：我们报告了在低温（<120摄氏度）下使用脉冲直流等离子体增强化学气相沉积沉积的类金刚石碳（DLC）和纳米晶体ZrO（2）的薄复合材料的合成。制备了含有最高21at。％Zr的薄膜（计算中不包括氢），并使用多种光谱方法和接触角测量方法确定了它们的结构和表面性质。使用成骨细胞进行为期3天的细胞培养，研究了骨细胞对薄膜的粘附性。这些纳米复合材料（DLC-ZrO（2））由平均尺寸为2-5 nm的四方ZrO（2）纳米晶体组成，嵌入到主要由DLC组成的非晶基质中。随着Zr含量从0原子％增加到21原子％，膜的表面水接触角从大约60度增加到80度。细胞培养研究表明，尽管细胞计数没有显着差异，但在DLC-ZrO（2）纳米复合材料上生长的成骨细胞的形态与仅在DLC上生长的细胞的形态明显不同。与在DLC表面上生长的细胞相比，在DLC-ZrO（2）表面上生长的细胞分布较少，并且细胞面积较小。在DLC-ZrO（2）表面上的某些区域，大量细胞似乎合并在一起。据推测，DLC-ZrO（2）表面和DLC表面上的成骨细胞之间的细胞形态差异与复合膜中ZrO（2）的非常小的四方纳米晶体的存在有关。

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