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首页> 外文期刊>Acta biomaterialia >Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process.
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Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process.

机译：使用组合的冻干/交联工艺制造多孔多糖基支架。

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摘要

Biocompatible three-dimensional (3-D) porous scaffolds are of great interest for tissue engineering applications. We here present a novel combined freeze-drying/cross-linking process to prepare porous polysaccharide-based scaffolds. This process does not require an organic solvent or porogen agent. We unexpectedly found that cross-linking of biomacromolecules such as pullulan and dextran with sodium trimetaphosphate could be performed during freeze-drying. We have demonstrated that the freeze-drying pressure modulates the degree of porosity. High freeze-drying pressure scaffolds presented pores with a mean diameter of 55 +/- 4 microm and a porosity of 33 +/- 12%, whereas low freeze-drying pressure scaffolds contained larger pores with a mean diameter of 243 +/- 14 microm and a porosity of 68 +/- 3%. Porous scaffolds of the desired shape could be easily obtained and were stable in culture medium for weeks. In vitro viable mesenchymal stem cells were found associated with porous scaffolds in higher proportions than with non-porous scaffolds. Moreover, cells penetrated deeper into scaffolds with larger pores. This novel combined freeze-drying/cross-linking processing of polysaccharides enabled the fabrication of biocompatible scaffolds with controlled porosity and architectures suitable for 3-D in vitro culture and biomedical applications.
机译：生物相容性三维（3-D）多孔支架对于组织工程应用非常感兴趣。我们在这里提出了一种新颖的组合的冷冻干燥/交联方法来制备基于多孔多糖的支架。该过程不需要有机溶剂或致孔剂。我们出乎意料地发现，在冷冻干燥过程中，可以将生物大分子（如支链淀粉和右旋糖酐）与三偏磷酸钠进行交联​​。我们已经证明冷冻干燥压力调节孔隙度。高冻干压力支架的孔平均直径为55 +/- 4微米，孔隙率为33 +/- 12％，而低冻干压力支架的孔更大，平均直径为243 +/- 14微米，孔隙率为68 +/- 3％。可以容易地获得所需形状的多孔支架，并且在培养基中稳定数周。发现体外存活的间充质干细胞与多孔支架相关的比例高于无孔支架。此外，细胞更深地渗透到具有较大孔的支架中。多糖的这种新颖的组合的冷冻干燥/交联处理使得能够制造具有受控孔隙率的生物相容性支架，并且其结构适合于3-D体外培养和生物医学应用。

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