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首页> 外文期刊>Acta biomaterialia >Candidate bone-tissue-engineered product based on human-bone-derived cells and polyurethane scaffold.
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Candidate bone-tissue-engineered product based on human-bone-derived cells and polyurethane scaffold.

机译：基于人骨细胞和聚氨酯支架的候选骨组织工程产品。

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Biodegradable polyurethanes (PURs) have recently been investigated as candidate materials for bone regenerative medicine. There are promising reports documenting the biocompatibility of selected PURs in vivo and the tolerance of certain cells toward PURs in vitro - potentially to be used as scaffolds for tissue-engineered products (TEPs). The aim of the present study was to take a step forward and create a TEP using human osteogenic cells and a polyurethane scaffold, and to evaluate the quality of the obtained TEP in vivo. Human-bone-derived cells (HBDCs) were seeded and cultured on polyurethane scaffolds in a bioreactor for 14 days. The TEP examination in vitro was based on the evaluation of cell number, cell phenotype and cell distribution within the scaffold. TEPs and control samples (scaffolds without cells) were implanted subcutaneously into SCID mice for 4 and 13 weeks. Explants harvested from the animals were examined using histological and immunohistochemical methods. They were also tested in mechanical trials. It was found that dynamic conditions for cell seeding and culture enable homogeneous distribution, maintaining the proliferative potential and osteogenic phenotype of the HBDCs cultured on polyurethane scaffolds. It was also found that HBDCs implanted as a component of TEP survived and kept their ability to produce the specific human bone extracellular matrix, which resulted in higher mechanical properties of the harvested explants when preseeded with HBDCs. The whole system, including the investigated PUR scaffold and the method of human cell seeding and culture, is recommended as a candidate bone TEP.
机译：最近已经研究了可生物降解的聚氨酯（PUR）作为骨再生医学的候选材料。有希望的报告记录了所选PUR在体内的生物相容性以及某些细胞在体外对PUR的耐受性-有望用作组织工程产品（TEP）的支架。本研究的目的是向前迈出一步，使用人成骨细胞和聚氨酯支架创建TEP，并评估体内获得的TEP的质量。接种人骨源细胞（HBDC），并在生物反应器中的聚氨酯支架上培养14天。体外TEP检查基于支架内细胞数量，细胞表型和细胞分布的评估。将TEP和对照样品（无细胞支架）皮下植入SCID小鼠中4和13周。使用组织学和免疫组织化学方法检查从动物收获的外植体。他们还进行了机械试验测试。已经发现，用于细胞接种和培养的动态条件能够实现均匀分布，从而维持在聚氨酯支架上培养的HBDC的增殖潜能和成骨表型。还发现作为TEP的一部分植入的HBDC幸存下来，并保持了产生特定人骨细胞外基质的能力，当与HBDC一起播种时，导致收获的外植体具有更高的机械性能。建议将整个系统（包括研究的PUR支架以及人类细胞接种和培养的方法）用作候选骨TEP。

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