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首页> 外文期刊>Acta biomaterialia >Three-dimensional poly(1,8-octanediol-co-citrate) scaffold pore shape and permeability effects on sub-cutaneous in vivo chondrogenesis using primary chondrocytes.
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Three-dimensional poly(1,8-octanediol-co-citrate) scaffold pore shape and permeability effects on sub-cutaneous in vivo chondrogenesis using primary chondrocytes.

机译：三维聚（1,8-辛二醇-柠檬酸）骨架孔的形状和通透性对使用原代软骨细胞的皮下体内软骨形成的影响。

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The objective of this study was to evaluate the coupled effects of three-dimensional poly(1,8-octanediol-co-citrate) (POC) scaffold pore shape and permeability on chondrogenesis using primary chondrocytes in vivo. Chondrogenesis was characterized as cartilage matrix formation by sulfated glycosaminoglycan (sGAG) quantification, relative mRNA expression of the cartilage-related proteins collagen types I, II and X, aggrecan and matrix metalloproteinases 13 and 3 and the compressive mechanical properties of the tissue/scaffold construct. A low permeability design with a spherical pore shape showed a significantly greater increase in cartilage matrix formation over 6 weeks in vivo than a high permeability design with a cubical pore shape. This increase in cartilage matrix synthesis corresponded with increases in mechanical compressive nonlinear elastic properties and histological data demonstrating darker red Safranin-O staining. There was higher mRNA expression for both cartilage-specific proteins and matrix degradation proteins in the high permeability design, resulting in overall less sGAG retained in the high permeability scaffold compared with the low permeability scaffold. Controlled POC scaffolds with a spherical pore shape and low permeability correlated with significantly increased cartilage matrix production using primary seeded chondrocytes. These results indicate that the low permeability design with a spherical pore shape provided a better microenvironment for chondrogenesis than the high permeability design with a cubical pore shape. Thus, scaffold architecture and material design may have a significant impact on the success of matrix-based clinical cartilage repair strategies.
机译：这项研究的目的是评估三维聚（1,8-辛二醇-柠檬酸-共柠檬酸酯）（POC）支架孔形状和通透性对体内使用原代软骨细胞的软骨形成的耦合作用。软骨形成的特征是通过硫酸化糖胺聚糖（sGAG）量化形成软骨基质，软骨相关蛋白I，II和X，软骨聚集蛋白聚糖和基质金属蛋白酶13和3的相对mRNA表达以及组织/支架构建体的压缩机械特性。与具有立方体孔形状的高渗透性设计相比，具有球形孔形状的低渗透性设计在体内六周内显示出软骨基质形成的显着更大的增加。软骨基质合成的这种增加与机械压缩非线性弹性性质和组织学数据的增加相对应，表明深红色的番红O-O染色。在高通透性设计中，软骨特异性蛋白和基质降解蛋白均具有更高的mRNA表达，与低通透性支架相比，高通透性支架中保留的sGAG总体较少。具有球形孔形状和低渗透性的受控POC支架与使用原种软骨细胞的软骨基质产生显着增加相关。这些结果表明，具有球形孔形状的低渗透性设计比具有立方体孔形状的高渗透性设计提供了更好的软骨形成微环境。因此，支架结构和材料设计可能对基于基质的临床软骨修复策略的成功产生重大影响。

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