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首页> 外文期刊>Acta biomaterialia >Cell type-specific responses of peripheral blood mononuclear cells to silver nanoparticles.
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Cell type-specific responses of peripheral blood mononuclear cells to silver nanoparticles.

机译：外周血单核细胞对银纳米颗粒的细胞类型特异性反应。

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Silver nanoparticles (Ag-NP) are increasingly used in biomedical applications because of their remarkable antimicrobial activity. In biomedicine, Ag-NP are coated onto or embedded in wound dressings, surgical instruments and bone substitute biomaterials, such as silver-containing calcium phosphate cements. Free Ag-NP and silver ions are released from these coatings or after the degradation of a biomaterial, and may come into close contact with blood cells. Despite the widespread use of Ag-NP as an antimicrobial agent, there is a serious lack of information on the biological effects of Ag-NP on human blood cells. In this study, the uptake of Ag-NP by peripheral monocytes and lymphocytes (T-cells) was analyzed, and the influence of nanosilver on cell biological functions (proliferation, the expression of adhesion molecules, cytokine release and the generation of reactive oxygen species) was studied. After cell culture in the presence of monodispersed Ag-NP (5-30mugml(-1) silver concentration), agglomerates of nanoparticles were detected within monocytes (CD14+) but not in T-cells (CD3+) by light microscopy, flow cytometry and combined focused ion beam/scanning electron microscopy. The uptake rate of nanoparticles was concentration dependent, and the silver agglomerates were typically found in the cytoplasm. Furthermore, a concentration-dependent activation (e.g. an increased expression of adhesion molecule CD54) of monocytes at Ag-NP concentrations of 10-15mugml(-1) was observed, and cytotoxicity of Ag-NP-treated monocytes was observed at Ag-NP levels of 25mugml(-1) and higher. However, no modulation of T-cell proliferation was observed in the presence of Ag-NP. Taken together, our results provide the first evidence for a cell-type-specific uptake of Ag-NP by peripheral blood mononuclear cells (PBMC) and the resultant cellular responses after exposure.
机译：银纳米颗粒（Ag-NP）由于其卓越的抗菌活性而越来越多地用于生物医学应用。在生物医学中，Ag-NP被涂覆或包埋在伤口敷料，手术器械和骨替代生物材料上，例如含银的磷酸钙水泥。游离的Ag-NP和银离子会从这些涂层中释放出来，或者在生物材料降解后释放出来，并可能与血细胞紧密接触。尽管Ag-NP被广泛用作抗微生物剂，但是仍然严重缺乏关于Ag-NP对人血细胞的生物学作用的信息。在这项研究中，分析了外周单核细胞和淋巴细胞（T细胞）对Ag-NP的吸收，以及纳米银对细胞生物学功能（增殖，粘附分子的表达，细胞因子释放和活性氧的产生）的影响。 ）进行了研究。在单分散Ag-NP（银浓度为5-30mugml（-1））存在下进行细胞培养后，通过光学显微镜，流式细胞仪检测并在单核细胞（CD14 +）中检测到纳米颗粒的团聚体，而在T细胞（CD3 +）中未检测到纳米颗粒的团聚体聚焦离子束/扫描电子显微镜。纳米颗粒的吸收速率是浓度依赖性的，并且通常在细胞质中发现银附聚物。此外，观察到浓度为10-15mugml（-1）的Ag-NP时单核细胞的浓度依赖性激活（例如粘附分子CD54表达增加），并且在Ag-NP处观察到经Ag-NP处理的单核细胞的细胞毒性。 25mugml（-1）和更高的水平。然而，在Ag-NP存在下未观察到T细胞增殖的调节。综上所述，我们的研究结果为外周血单核细胞（PBMC）对细胞类型的Ag-NP摄取以及暴露后产生的细胞反应提供了第一个证据。

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