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首页> 外文期刊>Acta biomaterialia >Conjugation of fibronectin onto three-dimensional porous scaffolds for vascular tissue engineering applications.
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Conjugation of fibronectin onto three-dimensional porous scaffolds for vascular tissue engineering applications.

机译：将纤连蛋白偶联到三维多孔支架上，用于血管组织工程应用。

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摘要

Tissue engineering scaffolds provide the three-dimensional (3-D) geometry and mechanical framework required for regulating cell behavior and facilitating tissue maturation. Unfortunately, most synthetic scaffolds lack the biological recognition motifs required for seeded cell interaction. In order to impart this recognition, synthetic scaffolds should possess appropriate biological functionality. Here, for the first time, we present a comprehensive study of fibronectin (FN) conjugation onto highly porous 3-D poly(carbonate) urethane scaffolds through grafted poly(acrylic acid) spacers on the urethane backbone. Scanning electron microscopy was used to ensure that the porous structures of the scaffolds were preserved throughout the multiple conjugation steps, and Fourier transform infrared spectroscopy was used to monitor the reaction progress. Toluidine blue staining revealed that increasing acrylic acid concentration and grafting time increased the number of poly(acrylic acid) groups incorporated. High resolution X-ray photoelectron spectroscopy studies of the scaffolds demonstrated an increase in nitrogen and sulfur due to FN conjugation. Immunofluorescence microscopy studies showed an even distribution of conjugated FN on the 3-D scaffolds. Cell culture studies using human coronary artery smooth muscle cells demonstrated that FN-conjugated scaffolds had improved cell attachment and infiltration depth compared with scaffolds without FN conjugation and with those scaffolds on which FN was merely adsorbed.
机译：组织工程支架提供了调节细胞行为和促进组织成熟所需的三维（3-D）几何形状和机械框架。不幸的是，大多数合成支架缺乏种子细胞相互作用所需的生物识别基序。为了给予这种认可，合成支架应具有适当的生物学功能。在这里，我们第一次对纤连蛋白（FN）通过氨基甲酸酯骨架上的接枝聚丙烯酸间隔基偶联到高度多孔的3-D聚碳酸酯氨基甲酸酯支架上进行了全面的研究。使用扫描电子显微镜确保支架的多孔结构在多个共轭步骤中得以保留，并使用傅立叶变换红外光谱法监测反应进程。甲苯胺蓝染色表明，增加丙烯酸浓度和接枝时间会增加并入的聚（丙烯酸）基团的数量。支架的高分辨率X射线光电子能谱研究表明，由于FN共轭，氮和硫增加。免疫荧光显微镜研究表明，共轭FN在3-D支架上的分布均匀。使用人冠状动脉平滑肌细胞进行的细胞培养研究表明，与不结合FN的支架和仅吸附FN的支架相比，结合FN的支架具有改善的细胞附着和浸润深度。

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