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首页> 外文期刊>Acta biomaterialia >Transport of biological molecules in surfactant-alginate composite hydrogels.
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Transport of biological molecules in surfactant-alginate composite hydrogels.

机译：表面活性剂-藻酸盐复合水凝胶中生物分子的运输。

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Obstructed transport of biological molecules can result in improper release of pharmaceuticals or biologics from biomedical devices. Recent studies have shown that nonionic surfactants, such as Pluronic(R) F68 (F68), positively alter biomaterial properties such as mesh size and microcapsule diameter. To further understand the effect of F68 (incorporated at concentrations well above the critical micelle concentration (CMC)) in traditional biomaterials, the transport properties of BSA and riboflavin were investigated in F68-alginate composite hydrogels, formed by both internal and external cross-linking with divalent cations. Results indicate that small molecule transport (represented by riboflavin) was not significantly hindered by F68 in homogeneously (internally) cross-linked hydrogels (up to an 11% decrease in loading capacity and 14% increase in effective diffusion coefficient, D(eff)), while protein transport in homogeneously cross-linked hydrogels (represented by BSA) was significantly affected (up to a 43% decrease in loading capacity and 40% increase in D(eff)). For inhomogeneously cross-linked hydrogels (externally cross-linked by CaCl(2) or BaCl(2)), the D(eff) increased up to 50 and 83% for small molecules and proteins, respectively. Variation in the alginate gelation method was shown to affect transport through measurable changes in swelling ratio (30% decrease) and observable changes in cross-linking structure as well as up to a 3.6- and 11.8-fold difference in D(eff) for riboflavin and BSA, respectively. Aside from the expected significant changes due to the cross-linking method utilized, protein transport properties were altered due to mesh size restrictions (10-25 nm estimated by mechanical properties) and BSA-F68 interaction (DLS). Taken as a whole, these results show that incorporation of a nonionic surfactant at concentrations above the CMC can affect device functionality by impeding the transport of large biological molecules.
机译：生物分子的运输受阻会导致药物或生物制剂从生物医学设备中释放不当。最近的研究表明，非离子表面活性剂，例如P68（F68）（F68），正积极改变生物材料的性能，例如筛孔尺寸和微胶囊直径。为了进一步了解F68（掺入浓度远高于临界胶束浓度（CMC）的浓度）在传统生物材料中的作用，研究了BSA和核黄素在F68-藻酸盐复合水凝胶中由内部和外部交联形成的转运特性。与二价阳离子。结果表明，F68在均质（内部）交联的水凝胶中并未明显阻碍小分子转运（以核黄素表示）（负载量最多降低11％，有效扩散系数D（eff）最多提高14％） ，而蛋白质在均质交联水凝胶（以BSA表示）中的转运受到显着影响（负载量最多降低43％，D（eff）最多提高40％）。对于非均质交联的水凝胶（通过CaCl（2）或BaCl（2）进行外部交联），对于小分子和蛋白质，D（eff）分别增加至50％和83％。藻酸盐胶凝方法的变化显示出通过可测量的溶胀率变化（降低30％）和可观察到的交联结构变化以及核黄素的D（eff）差异高达3.6和11.8倍来影响转运。和BSA分别。除了由于使用了交联方法而产生的预期显着变化之外，由于网孔尺寸的限制（通过机械性能估算为10-25 nm）和BSA-F68相互作用（DLS），蛋白质转运性能也发生了变化。总的来说，这些结果表明以高于CMC的浓度掺入非离子表面活性剂可通过阻止大生物分子的运输来影响设备功能。

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