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首页> 外文期刊>Acta biomaterialia >Insights into the mechanism of magnetic particle assisted gene delivery.
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Insights into the mechanism of magnetic particle assisted gene delivery.

机译：对磁性粒子辅助基因递送机制的见解。

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摘要

In magnetic particle assisted gene delivery DNA is complexed with polymer-coated aggregated magnetic nanoparticles (AMNPs) to effect transfection. In vitro studies based on COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT-complexed, polyethylenimine (PEI)-coated iron oxide magnetic nanoparticles (MNPs). PEI-coated AMNPs (PEI-AMNPs) with average individual particle diameters of 8, 16 and 30 nm were synthesized. Normal, reverse and retention magnetic transfection experiments and cell wounding assays were performed. Our results show that the optimum magnetic field yields maximum transfection efficiency with good viability. The results of the normal, reverse and retention magnetic transfection experiments show that the highest transfection efficiency was achieved in normal magnetic transfection mode due to clustering of the PEI-AMNPs on the cells. Cell wounding assay results suggest that the mechanism of magnetic transfection is endocytosis rather than cell wounding.
机译：在磁性粒子辅助的基因递送中，DNA与聚合物涂层的聚集磁性纳米粒子（AMNP）形成复合物以实现转染。使用pEGFP-N1和pMIR-REPORT复合的聚乙烯亚胺（PEI）涂层的氧化铁磁性纳米颗粒（MNP），进行了基于COS-7细胞的体外研究。合成了平均个体粒径为8、16和30 nm的PEI涂层AMNP（PEI-AMNP）。进行了正常，反向和保留磁性转染实验和细胞伤口测定。我们的结果表明，最佳磁场可产生最大转染效率并具有良好的生存能力。正常，反向和保留磁转染实验的结果表明，由于PEI-AMNPs在细胞上的聚集，在正常磁转染模式下实现了最高的转染效率。细胞创伤测定结果表明，磁性转染的机制是胞吞作用而不是细胞创伤。

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