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首页> 外文期刊>Acta biomaterialia >Base-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model
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Base-metal dental casting alloy biocompatibility assessment using a human-derived three-dimensional oral mucosal model

机译：基于人类三维口腔黏膜模型的贱金属牙科铸造合金生物相容性评估

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摘要

Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into the mechanisms of Ni-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto Alloderm? and maintained until full thickness was achieved prior to Ni-Cr and cobalt-chromium (Co-Cr) alloy disc exposure (2-72 h). Biocompatibility assessment involved histological analyses with cell viability measurements, oxidative stress responses, inflammatory cytokine expression and cellular toxicity analyses. Inductively coupled plasma mass spectrometry analysis determined elemental ion release levels. We detected adverse morphology with significant reductions in cell viability, significant increases in oxidative stress, inflammatory cytokine expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models compared with untreated oral mucosal models, and adverse effects were increased for the Ni-Cr alloy that leached the most Ni. Co-Cr demonstrated significantly enhanced biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-derived full-thickness oral mucosal model discriminated between dental alloys and provided insights into the mechanisms of Ni-induced toxicity, highlighting potential clinical relevance.
机译：固定修复术中使用的镍铬（Ni-Cr）合金与IV型镍诱发的超敏反应有关。我们假设，用于贱金属牙科合金生物相容性测试的全层人类来源口腔粘膜模型将提供有关Ni诱导毒性机制的见解。将原发性口腔角质形成细胞和牙龈成纤维细胞播种到Alloderm？并保持直至镍铬和钴铬（Co-Cr）合金圆盘暴露（2-72小时）之前达到全厚度。生物相容性评估涉及组织学分析，包括细胞活力测量，氧化应激反应，炎性细胞因子表达和细胞毒性分析。电感耦合等离子体质谱分析确定了元素离子的释放水平。与未治疗的口腔黏膜模型相比，我们发现Ni-Cr合金处理的口腔黏膜模型的不良形态具有明显的细胞活力降低，氧化应激，炎性细胞因子表达和细胞毒性显着增加，而对Ni-Cr合金的口腔黏膜模型则产生了不利影响Cr合金中Ni最多。与Ni-Cr合金治疗的口腔粘膜模型相比，Co-Cr表现出显着增强的生物相容性。源自人类的全厚度口腔粘膜模型可区分牙科合金，并提供了对镍诱导的毒性机制的见解，突出了潜在的临床意义。

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