• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Cell death in HeLa mediated by thermoplastic polyurethane with co-immobilized IFN-γ plus TNF-α
【24h】

Cell death in HeLa mediated by thermoplastic polyurethane with co-immobilized IFN-γ plus TNF-α

机译：热塑性聚氨酯与固定化的IFN-γ和TNF-α介导的HeLa细胞死亡

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to prohibit the toxicity of free IFN-γ plus TNF-α in treating human cervical cancer HeLa cells, two kinds of thermoplastic polyurethane (polyester/polyether) biomaterials with co-immobilized IFN-γ plus TNF-α on the surfaces are prepared. The programmed cell death of HeLa induced by these biomaterials is investigated. The surface modification of these biomaterials with co-immobilized IFN-γ plus TNF-α is performed by the photo-immobilization method, and the surface structures are characterized by various techniques. The cell morphology, cell mortality, cell cycle arrest, and functional status of caspases, upon the treatment by these biomaterials, are characterized. The results show that the as-prepared biomaterials have high inhibition activity against the growth of HeLa cells. The HeLa cells mediated by the two kinds of biomaterials are mainly arrested in the G 1 phase, while those cells mediated directly by free IFN-γ plus TNF-α are mainly arrested in the S phase. It is suggested that the programmed cell death mechanism induced by these two kinds of biomaterials is both caspase-dependent and caspase-independent. Our data provide the knowledge of microscopic surface structures and cell biology basis for synthesizing the thermoplastic polyurethane biomaterials with co-immobilized IFN-γ plus TNF-α, which are promising for novel therapeutics (e.g. drug cup) design for cervical cancer patients.
机译：为了抑制游离IFN-γ+TNF-α对人宫颈癌HeLa细胞的毒性作用，制备了两种表面共固定有IFN-γ+TNF-α的热塑性聚氨酯（聚酯/聚醚）生物材料。 。研究了由这些生物材料诱导的HeLa程序性细胞死亡。通过光固定化方法对这些生物材料进行共固定化的IFN-γ和TNF-α的表面修饰，并通过各种技术表征其表面结构。用这些生物材料处理后，表征了胱天蛋白酶的细胞形态，细胞死亡率，细胞周期停滞和功能状态。结果表明，所制备的生物材料对HeLa细胞的生长具有很高的抑制活性。两种生物材料介导的HeLa细胞主要滞留在G 1期，而游离IFN-γ和TNF-α直接介导的HeLa细胞主要滞留在S期。提示这两种生物材料诱导的程序性细胞死亡机制既依赖于caspase，又依赖于caspase。我们的数据提供了微观表面结构知识和细胞生物学基础，可用于将热塑性聚氨酯生物材料与固定化的IFN-γ和TNF-α一起合成，这对于宫颈癌患者的新型治疗方法（例如药杯）设计很有希望。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号