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首页> 外文期刊>Acta biomaterialia >Incorporation of bioactive polyvinylpyrrolidone-iodine within bilayered collagen scaffolds enhances the differentiation and subchondral osteogenesis of mesenchymal stem cells
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Incorporation of bioactive polyvinylpyrrolidone-iodine within bilayered collagen scaffolds enhances the differentiation and subchondral osteogenesis of mesenchymal stem cells

机译：在双层胶原支架中掺入生物活性聚乙烯吡咯烷酮-碘可增强间充质干细胞的分化和软骨下成骨

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摘要

Polyvinylpyrrolidone-iodine (Povidone-iodine, PVP-I) is widely used as an antiseptic agent for lavation during joint surgery; however, the biological effects of PVP-I on cells from joint tissue are unknown. This study examined the biocompatibility and biological effects of PVP-I on cells from joint tissue, with the aim of optimizing cell-scaffold based joint repair. Cells from joint tissue, including cartilage derived progenitor cells (CPC), subchondral bone derived osteoblast and bone marrow derived mesenchymal stem cells (BM-MSC) were isolated. The concentration-dependent effects of PVP-I on cell proliferation, migration and differentiation were evaluated. Additionally, the efficacy and mechanism of a PVP-I loaded bilayer collagen scaffold for osteochondral defect repair was investigated in a rabbit model. A micromolar concentration of PVP-I was found not to affect cell proliferation, CPC migration or extracellular matrix production. Interestingly, micromolar concentrations of PVP-I promote osteogenic differentiation of BM-MSC, as evidenced by up-regulation of RUNX2 and Osteocalcin gene expression, as well as increased mineralization on the three-dimensional scaffold. PVP-I treatment of collagen scaffolds significantly increased fibronectin binding onto the scaffold surface and collagen type I protein synthesis of cultured BM-MSC. Implantation of PVP-I treated collagen scaffolds into rabbit osteochondral defect significantly enhanced subchondral bone regeneration at 6 weeks post-surgery compared with the scaffold alone (subchondral bone histological score of 8.80 ± 1.64 vs. 3.8 ± 2.19, p < 0.05). The biocompatibility and pro-osteogenic activity of PVP-I on the cells from joint tissue and the enhanced subchondral bone formation in PVP-I treated scaffolds would thus indicate the potential of PVP-I for osteochondral defect repair.
机译：聚乙烯吡咯烷酮-碘（Povidone-iodine，PVP-1）被广泛用作关节手术中洗面的消毒剂;然而，PVP-1对关节组织细胞的生物学作用尚不清楚。这项研究检查了PVP-1对关节组织细胞的生物相容性和生物学作用，旨在优化基于细胞支架的关节修复。分离了来自关节组织的细胞，包括软骨衍生的祖细胞（CPC），软骨下骨衍生的成骨细胞和骨髓衍生的间充质干细胞（BM-MSC）。评价了PVP-1对细胞增殖，迁移和分化的浓度依赖性作用。另外，在兔模型中研究了载有PVP-1的双层胶原蛋白支架的骨软骨缺损修复的功效和机理。发现微摩尔浓度的PVP-1不影响细胞增殖，CPC迁移或细胞外基质产生。有趣的是，微摩尔浓度的PVP-1促进BM-MSC的成骨分化，这由RUNX2和Osteocalcin基因表达的上调以及在三维支架上矿化的增加证明。 PVP-I处理胶原蛋白支架明显增加了纤连蛋白与支架表面的结合以及培养的BM-MSC的I型胶原蛋白的合成。与单独使用支架相比，在术后6周将PVP-1治疗的胶原蛋白支架植入兔软骨软骨缺损显着增强了软骨下骨再生（软骨下骨组织学评分为8.80±1.64对3.8±2.19，p <0.05）。因此，PVP-1对关节组织细胞的生物相容性和促成骨活性以及经PVP-1处理的支架中软骨下骨形成的增强将表明PVP-1在骨软骨缺损修复中的潜力。

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