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首页> 外文期刊>Acta biomaterialia >Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies
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Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies

机译：脱细胞牛椎间盘作为异种细胞研究的天然支架

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Low back pain that is associated with disc degeneration contributes to a huge economic burden in the worldwide healthcare system. Traditional methods, such as spinal fusion, have been adopted to relieve mechanical back pain, but this is compromised by decreased spinal motion. Tissue engineering has attracted much attention, and aims to correct the changes fundamentally occurring in the discs by a combination of cell biology, molecular biology and engineering. Synthetic materials including poly(l-lactic acid) or poly(glycolic acid) and biomolecules like hyaluronic acid or collagen have been adopted in the development of disc scaffolds for studying therapeutic approaches. Nevertheless, the complex biological and mechanical environment of the intervertebral disc (IVD) makes the synthesis of an artificial IVD with biomaterials a difficult task. Thus the aim of this study was to develop a natural disc scaffold for culturing disc cells for future development of biological disc constructs. We adopted a combination of currently used decellularization techniques to decellularize bovine IVD to create a complete endplate-to-endplate IVD scaffold. By altering the chemical and physical decellularization parameters, we reported the removal of up to 70% of the endogenous cells, and were able to preserve the glycosaminoglycan content, collagen fibril architecture and mechanical properties of the discs. The reintroduction of nucleus pulposus cells into the scaffold indicated a high survival rate over 7 days, with cell penetration. We have shown here that conventional methods used for decellularizing thin tissues can also be applied to large organs, such as IVD. Our findings suggest the potential of using decellularized IVD as a scaffold for IVD bioengineering and culturing of cells in the context of the IVD niche. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：与椎间盘退变相关的腰背痛在全球医疗保健系统中造成巨大的经济负担。已经采用了诸如脊柱融合术之类的传统方法来减轻机械性背痛，但是由于脊柱运动的减少而受到损害。组织工程学引起了广泛的关注，其目的是通过结合细胞生物学，分子生物学和工程学来纠正光盘中根本发生的变化。在椎间盘支架的开发中已采用包括聚（1-乳酸）或聚（乙醇酸）以及生物分子（如透明质酸或胶原蛋白）的合成材料来研究治疗方法。然而，椎间盘（IVD）的复杂生物和机械环境使得用生物材料合成人工IVD成为一项艰巨的任务。因此，本研究的目的是开发用于培养椎间盘细胞的天然椎间盘支架，以用于将来的生物椎间盘构建物的开发。我们采用了当前使用的脱细胞技术的组合来对牛IVD脱细胞，以创建完整的终板到终板IVD支架。通过改变化学和物理脱细胞参数，我们报道了高达70％的内源性细胞被去除，并且能够保留糖胺聚糖含量，胶原纤维结构和椎间盘的机械性能。将髓核细胞重新引入支架表明在7天内具有较高的存活率，并且具有细胞穿透性。我们在这里已经表明，用于使薄组织脱细胞的常规方法也可以应用于大器官，例如IVD。我们的发现表明在IVD领域中，使用脱细胞的IVD作为IVD生物工程和细胞培养支架的潜力。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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