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首页> 外文期刊>Acta biomaterialia >Continuous gradient scaffolds for rapid screening of cell-material interactions and interfacial tissue regeneration
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Continuous gradient scaffolds for rapid screening of cell-material interactions and interfacial tissue regeneration

机译：连续梯度支架可快速筛选细胞-材料相互作用和界面组织再生

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In tissue engineering, the physical and chemical properties of the scaffold mediates cell behavior, including regeneration. Thus a strategy that permits rapid screening of cell-scaffold interactions is critical. Herein, we have prepared eight "hybrid" hydrogel scaffolds in the form of continuous gradients such that a single scaffold contains spatially varied properties. These scaffolds are based on combining an inorganic macromer (methacrylated star polydimethylsiloxane, PDMSstar-MA) and organic macromer (poly(ethylene glycol)diacrylate, PEG-DA) as well as both aqueous and organic fabrication solvents. Having previously demonstrated its bioactivity and osteoinductivity, PDMSstar-MA is a particularly powerful component to incorporate into instructive gradient scaffolds based on PEG-DA. The following parameters were varied to produce the different gradients or gradual transitions in: (1) the wt.% ratio of PDMSstar-MA to PEG-DA macromers, (2) the total wt.% macromer concentration, (3) the number average molecular weight (Mn) of PEG-DA and (4) the Mn of PDMSstar-MA. Upon dividing each scaffold into four "zones" perpendicular to the gradient, we were able to demonstrate the spatial variation in morphology, bioactivity, swelling and modulus. Among these gradient scaffolds are those in which swelling and modulus are conveniently decoupled. In addition to rapid screening of cell-material interactions, these scaffolds are well suited for regeneration of interfacial tissues (e.g. osteochondral tissues) that transition from one tissue type to another.
机译：在组织工程中，支架的物理和化学性质介导细胞行为，包括再生。因此，允许快速筛选细胞-支架相互作用的策略是至关重要的。在本文中，我们以连续梯度的形式制备了八个“混合”水凝胶支架，使得单个支架包含空间变化的特性。这些支架基于无机大分子单体（甲基丙烯酸酯化的星形聚二甲基硅氧烷，PDMSstar-MA）和有机大分子单体（聚（乙二醇）二丙烯酸酯，PEG-DA）以及水性和有机制造溶剂的组合。先前已证明其生物活性和骨诱导性，PDMSstar-MA是并入基于PEG-DA的指导性梯度支架中的特别强大的成分。更改以下参数以在以下方面产生不同的梯度或渐变：（1）PDMSstar-MA与PEG-DA大分子单体的重量％比率;（2）大分子单体的总重量％浓度;（3）数均PEG-DA的分子量（Mn）和（4）PDMSstar-MA的Mn。将每个支架分为垂直于梯度的四个“区域”后，我们能够证明形态，生物活性，膨胀和模量的空间变化。在这些梯度支架中，其中溶胀和模量方便地解耦的那些。除了快速筛选细胞-材料相互作用之外，这些支架还非常适合再生从一种组织类型过渡到另一种组织类型的界面组织（例如骨软骨组织）。

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