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首页> 外文期刊>Acta biomaterialia >Glycosaminoglycan mimetic peptide nanofibers promote mineralization by osteogenic cells
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Glycosaminoglycan mimetic peptide nanofibers promote mineralization by osteogenic cells

机译：糖胺聚糖模拟肽纳米纤维促进成骨细胞矿化

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摘要

Bone tissue regeneration is accomplished by concerted regulation of protein-based extracellular matrix components, glycosaminoglycans (GAGs) and inductive growth factors. GAGs constitute a significant portion of the extracellular matrix and have a significant impact on regulating cellular behavior, either directly or through encapsulation and presentation of growth factors to the cells. In this study we utilized a supramolecular peptide nanofiber system that can emulate both the nanofibrous architecture of collagenous extracellular matrix and the major chemical composition found on GAGs. GAGs and collagen mimetic peptide nanofibers were designed and synthesized with sulfonate and carboxylate groups on the peptide scaffold. The GAG mimetic peptide nanofibers interact with bone morphogenetic protein-2 (BMP-2), which is a critical growth factor for osteogenic activity. The GAG mimicking ability of the peptide nanofibers and their interaction with BMP-2 promoted osteogenic activity and mineralization by osteoblastic cells. Alkaline phosphatase activity, Alizarin red staining and energy dispersive X-ray analysis spectroscopy indicated the efficacy of the peptide nanofibers in inducing mineralization. The multifunctional and bioactive microenvironment presented here provides osteoblastic cells with osteogenic stimuli similar to those observed in native bone tissue.
机译：通过协调调节基于蛋白质的细胞外基质成分，糖胺聚糖（GAG）和诱导性生长因子，可以完成骨骼组织的再生。 GAG构成了细胞外基质的重要组成部分，对调节细胞行为具有重要影响，无论是直接作用还是通过将生长因子封装并呈递给细胞。在这项研究中，我们利用了超分子肽纳米纤维系统，该系统既可以模拟胶原细胞外基质的纳米纤维结构，也可以模拟在GAG上发现的主要化学成分。设计并合成了GAG和胶原蛋白模拟肽纳米纤维，并在肽支架上合成了具有磺酸根和羧基的基团。 GAG模拟肽纳米纤维与骨形态发生蛋白2（BMP-2）相互作用，而骨形态发生蛋白2是成骨活性的关键生长因子。肽纳米纤维的GAG模拟能力及其与BMP-2的相互作用促进了成骨细胞的成骨活性和矿化作用。碱性磷酸酶活性，茜素红染色和能量色散X射线分析光谱表明，肽纳米纤维在诱导矿化方面具有功效。此处介绍的多功能且具有生物活性的微环境为成骨细胞提供了与天然骨组织中观察到的相似的成骨性刺激。

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