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首页> 外文期刊>Acta biomaterialia >Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties
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Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties

机译：有机硅表面的抗菌功能化与工程化的短肽具有广谱的抗菌和耐盐性能

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摘要

Catheter-associated urinary tract infections (CAUTIs) are often preceded by pathogen colonization on catheter surfaces and are a major health threat facing hospitals worldwide. Antimicrobial peptides (AMPs) are a class of new antibiotics that hold promise in curbing CAUTIs caused by antibiotic-resistant pathogens. This study aims to systematically evaluate the feasibility of immobilizing two newly engineered arginine/lysine/tryptophan-rich AMPs with broad antimicrobial spectra and salt-tolerant properties on silicone surfaces to address CAUTIs. The peptides were successfully immobilized on polydimethylsiloxane and urinary catheter surfaces via an allyl glycidyl ether (AGE) polymer brush interlayer, as confirmed by X-ray photoelectron spectroscopy and water contact angle analyses. The peptide-coated silicone surfaces exhibited excellent microbial killing activity towards bacteria and fungi in urine and in phosphate-buffered saline. Although both the soluble and immobilized peptides demonstrated membrane disruption capabilities, the latter showed a slower rate of kill, presumably due to reduced diffusivity and flexibility resulting from conjugation to the polymer brush. The synergistic effects of the AGE polymer brush and AMPs prevented biofilm formation by repelling cell adhesion. The peptide-coated surface showed no toxicity towards smooth muscle cells. The findings of this study clearly indicate the potential for the development of AMP-based coating platforms to prevent CAUTIs.
机译：导管相关性尿路感染（CAUTI）通常在病原体定植在导管表面之前发生，是全世界医院面临的主要健康威胁。抗菌肽（AMPs）是一类新的抗生素，有望抑制由抗生素耐药性病原体引起的CAUTI。这项研究旨在系统地评估将两种新设计的精氨酸/赖氨酸/色氨酸丰富的AMP固定在有机硅表面上具有广泛的抗菌谱和耐盐性能以解决CAUTI的可行性。通过X射线光电子能谱和水接触角分析证实，这些肽已通过烯丙基缩水甘油醚（AGE）聚合物刷夹层成功固定在聚二甲基硅氧烷和导尿管表面上。肽涂层的有机硅表面对尿液和磷酸盐缓冲液中的细菌和真菌表现出出色的微生物杀灭活性。尽管可溶性和固定化肽均显示出膜破坏能力，但后者显示出较慢的杀灭速度，这可能是由于与聚合物刷结合产生的扩散性和柔韧性降低。 AGE聚合物刷和AMP的协同作用通过排斥细胞粘附阻止了生物膜的形成。肽包被的表面对平滑肌细胞无毒性。这项研究的结果清楚地表明了开发基于AMP的涂层平台以预防CAUTI的潜力。

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