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首页> 外文期刊>Acta biomaterialia >Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress.
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Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress.

机译：纳米模式对内皮细胞粘附的影响：剪切应力下增强的细胞保留。

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In this study, nanopatterned crosslinked films of collagen Type I were seeded with human microvascular endothelial cells and tested for their suitability for vascular tissue engineering. Since the films will be rolled into tubes with concentric layers of collagen, nutrient transfer through the collagen films is quite crucial. Molecular diffusivity through the collagen films, cell viability, cell proliferation and cell retention following shear stress were studied. Cells were seeded onto linearly nanogrooved films (groove widths of 332.5, 500 and 650nm), with the grooves aligned in the direction of flow. The nanopatterns did not affect cell proliferation or initial cell alignment; however, they significantly affected cell retention under fluid flow. While cell retention on unpatterned films was 35+/-10%, it was 75+/-4% on 332.5nm patterned films and even higher, 91+/-5%, on 650nm patterned films. The films were found to have diffusion coefficients of ca. 10(-6)cm(2)s(-1) for O(2) and 4-acetaminophenol, which is comparable to that observed in natural tissues. This constitutes another positive asset of these films for consideration as a scaffold material for vascular tissue engineering.
机译：在这项研究中，I型胶原蛋白的纳米图案交联膜被接种了人微血管内皮细胞，并测试了它们对血管组织工程的适用性。由于薄膜将被卷成带有同心胶原蛋白层的管子，因此通过胶原蛋白膜的营养转移非常关键。研究了在剪切应力作用下通过胶原膜的分子扩散性，细胞活力，细胞增殖和细胞保留。将细胞接种到线性纳米沟槽膜上（沟槽宽度为332.5、500和650nm），并使沟槽在流动方向上对齐。纳米图案不影响细胞增殖或初始细胞排列;然而，它们显着影响流体流动下的细胞滞留。虽然未图案化膜上的单元保留率为35 +/- 10％，但在332.5nm图案化膜上为75 +/- 4％，在650nm图案化膜上更高，为91 +/- 5％。发现该膜的扩散系数约为1。 O（2）和4-乙酰氨基苯酚的10（-6）cm（2）s（-1），与在自然组织中观察到的相当。这构成了这些薄膜的另一个积极资产，可以考虑用作血管组织工程的支架材料。

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