• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Bioactive membranes for bone regeneration applications: Effect of physical and biomolecular signals on mesenchymal stem cell behavior
【24h】

Bioactive membranes for bone regeneration applications: Effect of physical and biomolecular signals on mesenchymal stem cell behavior

机译：用于骨再生应用的生物活性膜：物理和生物分子信号对间充质干细胞行为的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study focuses on the in vitro characterization of bioactive elastin-like recombinamer (ELR) membranes for bone regeneration applications. Four bioactive ELRs exhibiting epitopes designed to promote mesenchymal stem cell adhesion (RGDS), endothelial cell adhesion (REDV), mineralization (HAP), and both cell adhesion and mineralization (HAP-RGDS) were synthesized using standard recombinant protein techniques. The materials were then used to fabricate ELR membranes incorporating a variety of topographical micropatterns including channels, holes and posts. Primary rat mesenchymal stem cells (rMSCs) were cultured on the different membranes and the effects of biomolecular and physical signals on cell adhesion, morphology, proliferation, and differentiation were evaluated. All results were analyzed using a custom-made MATLAB program for high throughput image analysis. Effects on cell morphology were mostly dependent on surface topography, while cell proliferation and cell differentiation were largely dependent on the biomolecular signaling from the ELR membranes. In particular, osteogenic differentiation (evaluated by staining for the osteoblastic marker osterix) was significantly enhanced on cells cultured on HAP membranes. Remarkably, cells growing on membranes containing the HAP sequence in non-osteogenic differentiation media exhibited significant up-regulation of the osteogenic marker as early as day 5, while those growing on fibronectin-coated glass in osteogenic differentiation media did not. These results are part of our ongoing effort to develop an optimized molecularly designed periosteal graft.
机译：这项研究的重点是用于骨再生应用的生物活性弹性蛋白样重组（ELR）膜的体外表征。使用标准重组蛋白技术合成了四个具有表位的生物活性ELR，这些表位旨在促进间充质干细胞粘附（RGDS），内皮细胞粘附（REDV），矿化（HAP）以及细胞粘附和矿化（HAP-RGDS）。然后将这些材料用于制造结合了各种地形微图案（包括通道，孔和柱）的ELR膜。在不同的膜上培养原代大鼠间充质干细胞（rMSCs），并评估生物分子和物理信号对细胞粘附，形态，增殖和分化的影响。使用定制的MATLAB程序对所有结果进行分析，以进行高通量图像分析。对细胞形态的影响主要取决于表面形貌，而细胞增殖和细胞分化则主要取决于来自ELR膜的生物分子信号传导。尤其是，在HAP膜上培养的细胞上，成骨分化（通过成骨细胞标志物osterix的染色评估）显着增强。值得注意的是，最早在第5天，在非成骨分化培养基中包含HAP序列的膜上生长的细胞就显示出明显的成骨标记物上调，而在成骨分化培养基中在纤连蛋白包被的玻璃上生长的细胞则没有。这些结果是我们为开发优化的分子设计的骨膜移植物而不断努力的一部分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号