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首页> 外文期刊>Acta biomaterialia >Poly(L-glutamic acid)/chitosan polyelectrolyte complex porous microspheres as cell microcarriers for cartilage regeneration
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Poly(L-glutamic acid)/chitosan polyelectrolyte complex porous microspheres as cell microcarriers for cartilage regeneration

机译：聚（L-谷氨酸）/壳聚糖聚电解质复合多孔微球作为软骨再生的细胞微载体

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In this study a novel kind of porous poly(l-glutamic acid) (PLGA)/chitosan polyelectrolyte complex (PEC) microsphere was developed through electrostatic interaction between PLGA and chitosan. By adjusting the formula parameters chitosan microspheres with an average pore size of 47.5 ± 5.4 μm were first developed at a concentration of 2 wt.% and freeze temperature of -20 C. For self-assembly of the PEC microspheres porous chitosan microspheres were then incubated in PLGA solution at 37 C. Due to electrostatic interaction a large amount of PLGA (110.3 μg mg-1) was homogeneously absorbed within the chitosan microspheres. The developed PEC microspheres retained their original size, pore diameters and interconnected porous structure. Fourier transform infrared spectroscopy, thermal gravimetric analysis and zeta potential analysis revealed that the PEC microspheres were successfully prepared through electrostatic interaction. Compared with microspheres fabricated from chitosan, the porous PEC microspheres were shown to efficiently promote chondrocyte attachment and proliferation. After injection subcutaneously for 8 weeks PEC microspheres loaded with chondrocytes were found to produce significant more cartilaginous matrix than chitosan microspheres. These results indicate that these novel fabricated porous PLGA/chitosan PEC microspheres could be used as injectable cell carriers for cartilage tissue engineering.
机译：在这项研究中，通过PLGA和壳聚糖之间的静电相互作用，开发了一种新型的多孔聚（1-谷氨酸）/壳聚糖聚电解质复合物（PEC）微球。通过调节配方参数，首先在2 wt。％的浓度和-20°C的冷冻温度下开发平均孔径为47.5±5.4μm的壳聚糖微球。为了自组装PEC微球，然后将多孔壳聚糖微球孵育在37 C的PLGA溶液中溶解。由于静电相互作用，大量的PLGA（110.3μgmg-1）被均匀吸收在壳聚糖微球中。发达的PEC微球保留了其原始尺寸，孔径和相互连接的多孔结构。傅里叶变换红外光谱，热重分析和ζ电势分析表明，PEC微球是通过静电作用成功制备的。与由壳聚糖制成的微球相比，多孔PEC微球显示出可有效促进软骨细胞的附着和增殖。皮下注射8周后，发现装有软骨细胞的PEC微球比壳聚糖微球可产生明显更多的软骨基质。这些结果表明，这些新颖的多孔PLGA /壳聚糖PEC微球可以用作软骨组织工程的可注射细胞载体。

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