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首页> 外文期刊>Acta biomaterialia >Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: Preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells
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Mussel-inspired bioceramics with self-assembled Ca-P/polydopamine composite nanolayer: Preparation, formation mechanism, improved cellular bioactivity and osteogenic differentiation of bone marrow stromal cells

机译：自组装Ca-P /聚多巴胺复合纳米层的贻贝启发生物陶瓷：骨髓基质细胞的制备，形成机理，改善的细胞生物活性和成骨分化

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The nanostructured surface of biomaterials plays an important role in improving their in vitro cellular bioactivity as well as stimulating in vivo tissue regeneration. Inspired by the mussel's adhesive versatility, which is thought to be due to the plaque-substrate interface being rich in 3,4-dihydroxy-l-phenylalamine (DOPA) and lysine amino acids, in this study we developed a self-assembly method to prepare a uniform calcium phosphate (Ca-P)/polydopamine composite nanolayer on the surface of β-tricalcium phosphate (β-TCP) bioceramics by soaking β-TCP bioceramics in Tris-dopamine solution. It was found that the addition of dopamine, reaction temperature and reaction time are three key factors inducing the formation of a uniform Ca-P/polydopamine composite nanolayer. The formation mechanism of a Ca-P/polydopamine composite nanolayer involved two important steps: (i) the addition of dopamine to Tris-HCl solution decreases the pH value and accelerates Ca and P ionic dissolution from the crystal boundaries of β-TCP ceramics; (ii) dopamine is polymerized to form self-assembled polydopamine film and, at the same time, nanosized Ca-P particles are mineralized with the assistance of polydopamine, in which the formation of polydopamine occurs simultaneously with Ca-P mineralization (formation of nanosized microparticles composed of calcium phosphate-based materials), and finally a self-assembled Ca-P/polydopamine composite nanolayer forms on the surface of the β-TCP ceramics. Furthermore, the formed self-assembled Ca-P/polydopamine composite nanolayer significantly enhances the surface roughness and hydrophilicity of β-TCP ceramics, and stimulates the attachment, proliferation, alkaline phosphate (ALP) activity and bone-related gene expression (ALP, OCN, COL1 and Runx2) of human bone marrow stromal cells. Our results suggest that the preparation of self-assembled Ca-P/polydopamine composite nanolayers is a viable method to modify the surface of biomaterials by significantly improving their surface physicochemical properties and cellular bioactivity for bone regeneration application.
机译：生物材料的纳米结构表面在改善其体外细胞生物活性以及刺激体内组织再生中起着重要作用。受贻贝胶粘剂多功能性的启发，据认为这是由于斑块-基质界面富含3,4-二羟基-1-苯丙胺（DOPA）和赖氨酸氨基酸，在这项研究中，我们开发了一种自组装方法通过将β-TCP生物陶瓷浸入Tris-多巴胺溶液中，在β-磷酸三钙（β-TCP）生物陶瓷表面制备均匀的磷酸钙（Ca-P）/聚多巴胺复合纳米层。发现多巴胺的添加，反应温度和反应时间是诱导形成均匀的Ca-P /聚多巴胺复合纳米层的三个关键因素。 Ca-P /聚多巴胺复合纳米层的形成机理涉及两个重要步骤：（i）将多巴胺添加到Tris-HCl溶液中会降低pH值，并加速Ca和P离子从β-TCP陶瓷的晶界溶解; （ii）使多巴胺聚合形成自组装的聚多巴胺薄膜，同时在聚多巴胺的辅助下将纳米级Ca-P颗粒矿化，其中聚多巴胺的形成与Ca-P矿化同时发生（形成纳米级由磷酸钙基材料组成的纳米微粒），最后在β-TCP陶瓷的表面形成自组装的Ca-P /聚多巴胺复合纳米层。此外，形成的自组装Ca-P /聚多巴胺复合纳米层显着提高了β-TCP陶瓷的表面粗糙度和亲水性，并刺激了附着，增殖，碱性磷酸酶（ALP）活性和骨相关基因表达（ALP，OCN） ，COL1和Runx2）。我们的结果表明，自组装Ca-P /聚多巴胺复合纳米层的制备是通过显着改善生物材料的表面理化特性和细胞生物活性进行骨再生应用来修饰生物材料表面的可行方法。

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