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首页> 外文期刊>Acta biomaterialia >In vitro assessment of three-dimensionally plotted nagelschmidtite bioceramic scaffolds with varied macropore morphologies
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In vitro assessment of three-dimensionally plotted nagelschmidtite bioceramic scaffolds with varied macropore morphologies

机译：三维绘制的具有不同大孔形态的钠闪石生物陶瓷支架的体外评估

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It is known that porous scaffolds play an important role in bone/periodontal tissue engineering. A new nagelschmidtite (NAGEL, Ca 7Si2P2O16) ceramic has recently been prepared which shows excellent apatite mineralization ability and osteo-/cementostimulation properties in vitro. However, up to now porous NAGEL scaffolds have not been developed yet. There has been no systematic study of the effect of macropore morphology of bioceramic scaffolds on their physico-chemical and biological properties. The aim of this study was to prepare NAGEL scaffolds for bone tissue engineering applications. We applied a modified three-dimensional (3-D) plotting method to prepare highly controllable NAGEL scaffolds and investigated the effect of macropore morphology on the physico-chemical and biological properties. The results showed that the macropore size and morphology of 3-D plotted NAGEL scaffolds could be effectively controlled. Compared with β-tricalcium phosphate (β-TCP) scaffolds NAGEL scaffolds possess a significantly enhanced compressive strength, a higher modulus and better degradability. Nagel scaffolds with a square pore morphology presented a higher compressive strength, a higher modulus and greater weight loss rate than those with triangular and parallelogram pore morphologies. In addition, all of the NAGEL scaffolds with the three macropore morphologies supported the attachment and proliferation of MC3T3 cells. The proliferation of MC3T3 cells on NAGEL scaffolds with triangular and parallelogram structures was higher than that on β-TCP scaffolds with the same pore structure. Cells on all three groups of NAGEL scaffolds revealed higher alkaline phosphatase (ALP) activity compared with cells on β-TCP scaffolds, and among the three NAGEL scaffolds groups those with a parallelogram pore structure showed the highest ALP activity. Furthermore, the angiogenic cell experiments showed that the ionic products from NAGEL scaffolds promoted tube formation, expression of pro-angiogenic factors and their receptors on human umbilical vein endothelial (HUVECs) compared with β-TCP scaffolds, indicating that NAGEL scaffolds possessed improved angiogenesis capacity. Our results suggest that 3-D plotted NAGEL scaffolds are a promising bioactive material for bone tissue engineering by virtue of their highly controllable macropore structure, excellent mechanical strength, degradability and in vitro biological response to osteogenic/angiogenic cells.
机译：已知多孔支架在骨/牙周组织工程中起重要作用。最近制备了一种新的钠辉石（NAGEL，Ca 7Si2P2O16）陶瓷，该陶瓷在体外显示出优异的磷灰石矿化能力和骨/骨水泥刺激特性。然而，到目前为止，尚未开发出多孔的NAGEL支架。尚未系统研究生物陶瓷支架的大孔形态对其理化和生物学特性的影响。这项研究的目的是为骨组织工程应用制备NAGEL支架。我们应用改进的三维（3-D）绘图方法来制备高度可控的NAGEL支架，并研究了大孔形态对理化和生物学特性的影响。结果表明3-D绘制的NAGEL支架的大孔尺寸和形态可以得到有效控制。与β-磷酸三钙（β-TCP）支架相比，NAGEL支架具有显着增强的抗压强度，更高的模量和更好的可降解性。与具有三角形和平行四边形孔形的那些相比，具有正方形孔形的Nagel支架具有更高的抗压强度，更高的模量和更大的失重率。此外，所有具有三种大孔形态的NAGEL支架均支持MC3T3细胞的附着和增殖。具有三角形和平行四边形结构的NAGEL支架上的MC3T3细胞的增殖高于具有相同孔结构的β-TCP支架上的MC3T3细胞的增殖。三组NAGEL支架上的细胞均比β-TCP支架上的细胞具有更高的碱性磷酸酶（ALP）活性，而三个NAGEL支架组中具有平行四边形孔结构的细胞显示出最高的ALP活性。此外，血管生成细胞实验表明，与β-TCP支架相比，NAGEL支架的离子产物可促进管形成，促血管生成因子及其受体在人脐静脉内皮（HUVEC）上的表达，表明NAGEL支架具有改善的血管生成能力。我们的结果表明3-D绘图NAGEL支架凭借其高度可控的大孔结构，出色的机械强度，可降解性以及对成骨/血管生成细胞的体外生物学响应，是用于骨组织工程的有前途的生物活性材料。

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