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首页> 外文期刊>Acta biomaterialia >Gene expression profile on chitosan/rhBMP-2 films: A novel osteoinductive coating for implantable materials.
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Gene expression profile on chitosan/rhBMP-2 films: A novel osteoinductive coating for implantable materials.

机译：壳聚糖/ rhBMP-2膜上的基因表达谱：用于植入材料的新型骨诱导涂层。

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摘要

This study focusses on the gene expression profile related to a new rhBMP-2 carrier material, chitosan film. This film could be suitable for use as an osteoinductive coating of commercially available titanium implants. The developed material was characterized, biocompatibility was tested and the cellular response was extensively characterized by transcriptional expression studies. Finally, in vivo studies were carried out to confirm the osteoinductivity of the developed coating. Results show good material properties for cell adhesion and proliferation. Presented data show cellular differentiation to the osteoblastic phenotype due to rhBMP-2, with a 90% common transcriptional response between the control rhBMP-2 treatment and the developed chitosan/rhBMP-2 film. The growing surface also had an influence on the observed cellular response and was quantified as 7% of the total. These results indicate that both the growth factor and the material induce a cell response, but this is mainly driven by the osteoinductor factor. In vivo, new bone formation and early vascularization was observed around chitosan/rhBMP-2 coated titanium pieces implanted in mouse muscle. In contrast, control implants did not induce this reaction. This work, therefore, shows both in vitro and in vivo that chitosan/rhBMP-2 film is a promising osteoinductive coating for titanium implantable materials.
机译：这项研究的重点是与新型rhBMP-2载体材料壳聚糖膜有关的基因表达谱。该膜可能适合用作市售钛植入物的骨诱导涂层。表征了开发的材料，测试了生物相容性，并通过转录表达研究广泛表征了细胞反应。最后，进行了体内研究以确认显影涂层的骨诱导性。结果显示了用于细胞粘附和增殖的良好材料性能。提出的数据显示由于rhBMP-2，细胞分化为成骨细胞表型，在对照rhBMP-2处理和已开发的壳聚糖/ rhBMP-2膜之间有90％的常见转录反应。生长的表面也对观察到的细胞反应有影响，被定量为总数的7％。这些结果表明，生长因子和材料均诱导细胞反应，但这主要是由骨诱导因子驱动的。在体内，在植入小鼠肌肉的壳聚糖/ rhBMP-2涂层钛片周围观察到新的骨形成和早期血管形成。相反，对照植入物没有引起该反应。因此，这项工作在体外和体内均显示壳聚糖/ rhBMP-2膜是用于钛可植入材料的有希望的骨诱导涂层。

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