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首页> 外文期刊>Acta biomaterialia >Photopatterned collagen-hyaluronic acid interpenetrating polymer network hydrogels.
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Photopatterned collagen-hyaluronic acid interpenetrating polymer network hydrogels.

机译：光致图案化的胶原蛋白-透明质酸互穿聚合物网络水凝胶。

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To engineer complex tissues, it is necessary to create hybrid scaffolds with micropatterned structural and biomechanical properties, which can closely mimic the intricate body tissues. The current report describes the synthesis of a novel photocrosslinkable interpenetrating polymeric network (IPN) of collagen and hyaluronic acid (HA) with precisely controlled structural and biomechanical properties. Both collagen and HA are present in crosslinked form in IPNs, and the two networks are entangled with each other. IPNs were also compared with semi-IPNs (SIPN), in which only collagen was in network form and HA chains were entangled in the collagen network without being photocrosslinked. Scanning electron microscopy images revealed that IPNs are denser than SIPNs, which results in their molecular reinforcement. This was further confirmed by rheological experiments. Because of the presence of the HA crosslinked network, the storage modulus of IPNs was almost two orders of magnitude higher than SIPNs. The degradation of the collagen-HA IPNs was slower than the SIPNs because of the presence of the crosslinked HA network. Increasing concentration of HA further altered the properties among IPNs. Cytocompatibility of IPNs was confirmed by Schwann cell and dermal fibroblasts adhesion and proliferation studies. We also fabricated patterned scaffolds with regions of IPNs and SIPNs within a bulk hydrogel, resulting in zonal distribution of crosslinking densities, viscoelasticities, water content and pore sizes at the micro- and macro-scales. With the ability to fine-tune the scaffold properties by performing structural modifications and to create patterned scaffolds, these hydrogels can be employed as potential candidates for regenerative medicine applications.
机译：为了工程化复杂的组织，有必要创建具有微图案结构和生物力学特性的杂种支架，这些支架可以紧密模仿复杂的身体组织。本报告描述了具有精确控制的结构和生物力学特性的新型胶原和透明质酸（HA）的可光交联互穿聚合物网络（IPN）的合成。胶原蛋白和HA均以交联形式存在于IPN中，并且这两个网络相互缠绕。还将IPN与半IPN（SIPN）进行了比较，SIPN中只有胶原蛋白呈网络形式，HA链缠绕在胶原蛋白网络中而未进行光交联。扫描电子显微镜图像显示IPN比SIPN致密，这导致它们的分子增强。流变实验进一步证实了这一点。由于HA交联网络的存在，IPN的存储模量比SIPN几乎高两个数量级。由于存在交联的HA网络，胶原-HA IPN的降解比SIPN慢。 HA浓度的增加进一步改变了IPN之间的特性。通过雪旺氏细胞和皮肤成纤维细胞的粘附和增殖研究证实了IPN的细胞相容性。我们还制造了在整体水凝胶中具有IPN和SIPN区域的图案化支架，从而在微观和宏观尺度上产生了交联密度，粘弹性，水含量和孔径的区域分布。这些水凝胶具有通过执行结构修饰来微调支架特性并创建带图案的支架的能力，可以用作再生医学应用的潜在候选者。

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