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首页> 外文期刊>Acta biomaterialia >Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: cellular adhesiveness and feasibility for cell sheet technology.
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Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: cellular adhesiveness and feasibility for cell sheet technology.

机译：酶促交联的羧甲基纤维素-酪胺共轭水凝胶：细胞粘附性和细胞片技术的可行性。

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摘要

An aqueous solution of carboxylmethylcellulose with phenolic hydroxyl groups (CMC-Ph) is gellable within 1 min via a peroxidase-catalyzed oxidative reaction under mild conditions suitable for mammalian cells. In this research, we evaluated cellular adhesion and proliferation on the resultant hydrogel, and the feasibility of the hydrogel as a substrate for cell sheet technology. Within 4 h of seeding, 76.9% of L929 fibroblast cells adhered to the gel and showed similar morphology of spreading to that on cell culture dish. Subsequently, the adherent cells proliferated on the gel and formed a confluent monolayer after 168 h of culture. From the confluent monolayer we could harvest a cell sheet after about 5 min of digestion of the gel using cellulase dissolved in medium at 5 U ml(-1). The cells in the cell sheet showed well-preserved morphology similar to that shown before they were detached from the gel. In addition, the harvested cell sheet readhered and proliferated after being transferred to another culture dish. These results demonstrate that CMC-Ph gel is a good candidate material for obtaining cell sheets.
机译：具有酚羟基的羧甲基纤维素水溶液（CMC-Ph）在适合哺乳动物细胞的温和条件下，可通过过氧化物酶催化的氧化反应在1分钟内胶凝。在这项研究中，我们评估了在最终水凝胶上的细胞粘附和增殖，以及水凝胶作为细胞片技术基质的可行性。接种后4小时内，有76.9％的L929成纤维细胞粘附在凝胶上，并表现出与细胞培养皿相似的铺展形态。随后，贴壁细胞在培养168小时后在凝胶上增殖并形成融合的单层。从融合的单层中，我们可以使用溶解在5 U ml（-1）培养基中的纤维素酶消化凝胶约5分钟后，收获细胞片。细胞片中的细胞显示出良好保存的形态，类似于从凝胶上分离之前显示的形态。另外，将收获的细胞片转移到另一个培养皿中后再增殖并增殖。这些结果表明，CMC-Ph凝胶是获得细胞片的良好候选材料。

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