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首页> 外文期刊>Acta biomaterialia >Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation.
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Electroconductive polymeric nanowire templates facilitates in vitro C17.2 neural stem cell line adhesion, proliferation and differentiation.

机译：导电聚合物纳米线模板可促进体外C17.2神经干细胞系的粘附，增殖和分化。

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摘要

Stem cells still remain one of the most exciting and lucrative options for treatment of a variety of nervous system disorders and diseases. Although there are neural stem cells present in adults, the ability of both the peripheral and central nervous system for self-repair is limited at best. As such, there is a great need for a tissue engineering approach to solve nervous system disorders and diseases. In this study, we have developed electrically conductive surfaces with controlled arrays of high aspect ratio nanowires for the growth and maintenance of neural stem cells. The nanowire surfaces were fabricated from polycaprolactone using a novel nanotemplating technique, and were coated with an electrically conductive polymer, polypyrrole. The polypyrrole-coated nanowire surfaces were characterized using scanning electron microscopy and X-ray photoelectron spectroscopy. Additionally, the surface resistance of polypyrrole-coated nanowire surfaces was measured. C17.2 neural stem cells were used to evaluate the efficacy of the polypyrrole-coated nanowire surfaces to promote cell adhesion, proliferation and differentiation. The results presented here indicate significantly higher cellular adhesion and proliferation on polypyrrole-coated nanowire surfaces as compared to control surfaces. The differentiation potential of polypyrrole nanowire surfaces was also evaluated by immunostaining key neuronal markers that are expressed when NSCs differentiate into their respective neural lineages.
机译：干细胞仍然是治疗各种神经系统疾病的最令人兴奋和最有利可图的选择之一。尽管成人中存在神经干细胞，但充其量，外周和中枢神经系统的自我修复能力受到最大限制。因此，非常需要用于解决神经系统疾病和疾病的组织工程方法。在这项研究中，我们开发了具有高纵横比纳米线受控阵列的导电表面，用于神经干细胞的生长和维持。纳米线表面是使用新型的纳米模板技术由聚己内酯制成的，并涂有导电聚合物聚吡咯。使用扫描电子显微镜和X射线光电子能谱表征聚吡咯涂层的纳米线表面。另外，测量了涂覆有聚吡咯的纳米线表面的表面电阻。 C17.2神经干细胞用于评估聚吡咯涂层纳米线表面促进细胞粘附，增殖和分化的功效。此处呈现的结果表明，与对照表面相比，聚吡咯涂层纳米线表面的细胞粘附和增殖明显更高。还通过免疫染色关键神经元标记物评估了聚吡咯纳米线表面的分化潜能，这些标记物在NSC分化为各自的神经谱系时表达。

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