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首页> 外文期刊>Acta biomaterialia >Aligned silk-based 3-D architectures for contact guidance in tissue engineering
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Aligned silk-based 3-D architectures for contact guidance in tissue engineering

机译：对齐的基于丝绸的3-D架构，用于组织工程中的接触指导

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摘要

An important challenge in the biomaterials field is to mimic the structure of functional tissues via cell and extracellular matrix (ECM) alignment and anisotropy. Toward this goal, silk-based scaffolds resembling bone lamellar structure were developed using a freeze-drying technique. The structure could be controlled directly by solute concentration and freezing parameters, resulting in lamellar scaffolds with regular morphology. Different post-treatments, such as methanol, water annealing and steam sterilization, were investigated to induce water stability. The resulting structures exhibited significant differences in terms of morphological integrity, structure and mechanical properties. The lamellar thicknesses were ～2.6 μm for the methanol-treated scaffolds and ～5.8 μm for water-annealed. These values are in the range of those reported for human lamellar bone. Human bone marrow-derived mesenchymal stem cells (hMSC) were seeded on these silk fibroin lamellar scaffolds and grown under osteogenic conditions to assess the effect of the microstructure on cell behavior. Collagen in the newly deposited ECM was found aligned along the lamellar architectures. In the case of methanol-treated lamellar structures, the hMSC were able to migrate into the interior of the scaffolds, producing a multilamellar hybrid construct. The present morphology constitutes a useful pattern onto which hMSC cells attach and proliferate for guided formation of a highly oriented extracellular matrix.
机译：生物材料领域的一个重要挑战是通过细胞和细胞外基质（ECM）的排列和各向异性来模仿功能组织的结构。为了实现这个目标，使用冷冻干燥技术开发了类似骨层状结构的丝绸基支架。可以直接通过溶质浓度和冷冻参数来控制结构，从而形成具有规则形态的层状支架。研究了不同的后处理，例如甲醇，水退火和蒸汽灭菌，以诱导水的稳定性。所得结构在形态完整性，结构和机械性能方面表现出显着差异。甲醇处理的支架的层状厚度约为2.6μm，水退火的层状厚度约为5.8μm。这些值在报道的人类层状骨的范围内。将人骨髓源间充质干细胞（hMSC）播种在这些丝素蛋白层状支架上，并在成骨条件下生长，以评估微结构对细胞行为的影响。发现新沉积的ECM中的胶原蛋白沿层状结构排列。在用甲醇处理的层状结构的情况下，hMSC能够迁移到支架内部，从而产生多层混合构建体。本形态构成了有用的模式，hMSC细胞附着并增殖以指导高度定向的细胞外基质的形成。

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