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首页> 外文期刊>Acta biomaterialia >Excimer laser channel creation in polyethersulfone hollow fibers for compartmentalized in vitro neuronal cell culture scaffolds.
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Excimer laser channel creation in polyethersulfone hollow fibers for compartmentalized in vitro neuronal cell culture scaffolds.

机译：准分子激光通道在聚醚砜中空纤维中用于分隔的体外神经元细胞培养支架。

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摘要

Hollow fiber scaffolds that compartmentalize axonal processes from their cell bodies can enable neuronal cultures with directed neurite outgrowth within a three-dimensional (3-D) space for controlling neuronal cell networking in vitro. Controllable 3-D neuronal networks in vitro could provide tools for studying neurobiological events. In order to create such a scaffold, polyethersulfone (PES) microporous hollow fibers were ablated with a KrF excimer laser to generate specifically designed channels for directing neurite outgrowth into the luminal compartments of the fibers. Excimer laser modification is demonstrated as a reproducible method to generate 5microm diameter channels within PES hollow fiber walls that allow compartmentalization of neuronal cell bodies from their axons. Laser modification of counterpart flat sheet PES membranes with peak surface fluences of 1.2Jcm(-2) results in increased hydrophobicity and laminin adsorption on the surface compared with the unmodified PES surface. This is correlated to enhanced PC12 cell adhesion with increasing fluence onto laser-modified PES membrane surfaces coated with laminin when compared with unmodified surfaces. Adult rat neural progenitor cells differentiated on PES fibers with laser-created channels resulted in spontaneous cell process growth into the channels of the scaffold wall while preventing entrance of cell bodies. Therefore, laser-modified PES fibers serve as scaffolds with channels conducive to directing neuronal cell process growth. These hollow fiber scaffolds can potentially be used in combination with perfusion and oxygenation hollow fiber membrane sets to construct a hollow fiber-based 3-D bioreactor for controlling and studying in vitro neuronal networking in three dimensions between compartmentalized cultures.
机译：中空的轴突过程从其细胞体分隔的空心纤维支架可以使具有定向神经突生长的神经元培养物在三维（3-D）空间内控制体外的神经元细胞网络连接。体外可控的3-D神经元网络可以为研究神经生物学事件提供工具。为了产生这样的支架，用KrF准分子激光烧蚀聚醚砜（PES）微孔中空纤维，以产生专门设计的通道，用于将神经突向外生长引入纤维的腔室中。准分子激光修饰被证明是可重现的方法，可在PES中空纤维壁内产生5微米直径的通道，从而允许神经元细胞体从其轴突区分开。与未改性的PES表面相比，激光改性的对等平板PES膜的峰表面通量为1.2Jcm（-2），导致疏水性和层粘连蛋白在表面上的吸附增加。与未修饰的表面相比，这与PC12细胞粘附力的增强有关，并增加了在层粘连蛋白涂层的激光修饰的PES膜表面的通量。在具有激光创建通道的PES纤维上分化的成年大鼠神经祖细胞导致自发的细胞过程生长进入支架壁的通道，同时阻止细胞体进入。因此，激光改性的PES纤维可作为具有引导神经元细胞过程生长的通道的支架。这些中空纤维支架可潜在地与灌注和氧合中空纤维膜组结合使用，以构建基于中空纤维的3-D生物反应器，用于在分隔培养物之间的三个维度上控制和研究体外神经元网络。

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