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首页> 外文期刊>Acta biomaterialia >Neu5Ac??2,6Gal and Neu5Ac??2,3Gal receptor specificities on influenza viruses determined by a waveguide-mode sensor
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Neu5Ac??2,6Gal and Neu5Ac??2,3Gal receptor specificities on influenza viruses determined by a waveguide-mode sensor

机译：通过波导模式传感器确定的Neu5Ac ?? 2,6Gal和Neu5Ac ?? 2,3Gal受体对流感病毒的特异性

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摘要

To characterize the differences in the receptor-binding specificities of human and avian influenza viruses with glycan chains, the authors performed binding analyses using an evanescent field-coupled waveguide-mode biosensor. The experiments were performed on intact viruses and hemagglutinin proteins, using gold-nanoparticle-conjugated Neu5Ac??2,6Gal and Neu5Ac??2,3Gal glycan chains. Several influenza viruses belonging to subtypes H3N2 (A/Udorn/307/1972, A/Shandong/9/1993, A/Kiev/301/1994, A/Panama/2007/1999, A/Wisconsin/67/2005 and A/Brisbane/10/2007), H1N1 (A/Brisbane/59/2007 and A/California/07/2009) and H5N1 (A/chicken/India/NIV33487/2006) were used. High levels of glycan-based discrimination were observed with the H3N2 strain A/Brisbane/10/2007 due to its specificity with Neu5Ac??2,6Gal, but not with Neu5Ac??2,3Gal. Possible amino acid residues responsible for the discrimination of human and avian influenza viruses are discussed. These types of sensor-based discriminatory analyses would be very useful for distinguishing between influenza pandemics, especially during the transition and overlapping periods of human and avian influenza viruses with evolutionary changes. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：为了表征人类和禽流感病毒具有聚糖链的受体结合特异性的差异，作者使用an逝场耦合波导模式生物传感器进行了结合分析。用金-纳米颗粒结合的Neu5Ac 12，2，6Gal和Neu5Ac 12，2，3Gal聚糖链对完整的病毒和血凝素蛋白进行了实验。属于H3N2亚型的几种流感病毒（A / Udorn / 307/1972，A / Shandong / 9/1993，A / Kiev / 301/1994，A / Panama / 2007/1999，A / Wisconsin / 67/2005和A /使用了布里斯班（Brisbane / 10/2007），H1N1（A / Brisbane / 59/2007和A / California / 07/2009）和H5N1（A / chicken / India / NIV33487 / 2006）。 H3N2菌株A / Brisbane / 10/2007观察到基于聚糖的高水平区分，这是由于其对Neu5AcΔ2,6Gal具有特异性，而对Neu5AcΔ2,3Gal则无特异性。讨论了可能区分人和禽流感病毒的可能的氨基酸残基。这些类型的基于传感器的歧视性分析对于区分流感大流行非常有用，尤其是在人类和禽流感病毒具有进化变化的过渡期和重叠期。 ？ 2012年Acta Materialia Inc.由Elsevier Ltd.发行。保留所有权利。

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