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首页> 外文期刊>Acta biomaterialia >Shape-memory-actuated change in scaffold fiber alignment directs stem cell morphology
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Shape-memory-actuated change in scaffold fiber alignment directs stem cell morphology

机译：形状记忆驱动的支架纤维排列方式变化指导干细胞形态

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Tissue engineering scaffolds have traditionally been static physical structures poorly suited to mimicking the complex dynamic behavior of in vivo microenvironments. Here we present a thermoresponsive scaffold that can be programmed to change macroscopic shape and microscopic architecture during cell culture. The scaffold, which was prepared by electrospinning a shape memory polymer (SMP), was used to test the hypothesis that a shape-memory-actuated change in scaffold fiber alignment could be used to control the behavior of attached and viable cells. To test this hypothesis, we stretched an SMP scaffold of randomly oriented fibers and fixed the scaffold in a temporary but stable elongated shape in which fibers were aligned by the strain. Following seeding and culture of human adipose-derived stem cells on the strain-aligned scaffold, the scaffold was triggered to transition back to its initial shape and random fiber orientation via shape memory actuation using a cytocompatible temperature increase. We found that cells preferentially aligned along the fiber direction of the strain-aligned scaffold before shape memory actuation. After shape memory actuation, cells remained attached and viable but lost preferential alignment. These results demonstrate that shape-memory-actuated changes in scaffold fiber alignment can be achieved with attached and viable cells and can control cell morphological behavior. The incorporation of shape memory into cytocompatible scaffolds is anticipated to facilitate the development, delivery and functionality of tissue engineering scaffolds and the in vitro and in vivo study and application of mechanobiology.
机译：传统上，组织工程支架是静态的物理结构，不适合模仿体内微环境的复杂动态行为。在这里，我们介绍了一种热响应支架，可以对其进行编程以在细胞培养过程中改变宏观形状和微观结构。通过静电纺丝形状记忆聚合物（SMP）制备的支架用于测试以下假设：支架记忆体排列中的形状记忆驱动变化可用于控制附着细胞和存活细胞的行为。为了检验该假设，我们拉伸了随机取向纤维的SMP支架，并将其固定在临时但稳定的细长形状中，其中纤维通过应变对齐。在应变对齐的支架上播种和培养人脂肪来源的干细胞后，通过使用细胞相容性温度升高，通过形状记忆激活，触发支架转变回其初始形状和随机纤维取向。我们发现，在形状记忆激活之前，细胞优先沿着应变排列支架的纤维方向排列。激活形状记忆后，细胞保持附着并存活，但失去优先排列。这些结果表明，形状记忆驱动的支架纤维排列方式的变化可以通过附着和存活的细胞来实现，并且可以控制细胞的形态行为。预期将形状记忆结合到细胞相容性支架中将促进组织工程支架的开发，递送和功能性以及机械生物学的体外和体内研究及应用。

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