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首页> 外文期刊>RSC Advances >A new approach to the determination of folic acid at trace levels: using a Fe(III)-folic acid complex to amplify analytical signal
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A new approach to the determination of folic acid at trace levels: using a Fe(III)-folic acid complex to amplify analytical signal

机译：痕量水平测定叶酸的新方法：使用Fe（III） - 酸综合体扩增分析信号

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摘要

A fast, efficient, cost-effective, and environmental-friendly analytical methodology was developed for preconcentration and determination of trace folic acid in food samples prior to high performance liquid chromatography with diode array detection (HPLC-DAD). The method is based on formation of stable complexes between folic acid and Fe(III) ions at pH 8.0. The formed complexes were extracted to a nonionic surfactant phase containing PONPE 7.5. The surfactant rich phase (SRP) was separated by decantation and diluted with 300 mu L of a mixture of 1 M HCl and methanol at a 1 : 1 ratio. The parameters and variables that affected the method were also investigated and optimized in detail. The limit of detection (LOD) of folic acid was 6.06 ng mL(-1), the linear range of quantitation for folic acid was 20-1200 ng mL(-1) and the correlation coefficients of the calibration curves were 0.9976. The average recoveries and relative standard deviations in the analysis of real samples were in the range of 95.1-105.1% and 1.73-5.25%, respectively. After validation of the method was carried out, the method was applied to the determination of folic acid in real samples, including baby foods, vegetables, cereals, and pharmaceutical samples.
机译：开发了一种快速，高效，具有成本效益和环保的分析方法，用于预浓度和测定在高性能液相色谱法与二极管阵列检测（HPLC-DAD）之前的食物样品中的痕量叶酸。该方法基于在pH8.0下形成叶酸和Fe（III）离子之间的稳定复合物。将形成的配合物萃取到含有PONPE 7.5的非离子表面活性剂相。表面活性剂富相（SRP）通过倾析分离并用300μl的1M HCl和甲醇的混合物稀释，1：1的比例。影响了该方法的参数和变量也详细研究和优化。叶酸的检测极限为6.06 ng ml（-1），叶酸的线性定量范围为20-1200ng ml（-1），校准曲线的相关系数为0.9976。实际样品分析中的平均回收率和相对标准偏差分别为95.1-105.1％和1.73-5.25％。在进行该方法验证后，将该方法应用于真实样品中叶酸的测定，包括婴儿食品，蔬菜，谷物和药物样品。

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  • 来源
    《RSC Advances》 |2016年第46期|共8页
  • 作者

    Ulusoy Songul; Acidereli Hilal; Erdogan Selim; Ulusoy Halil Ibrahim;

  • 作者单位

    Cumhuriyet Univ Fac Technol Dept Biomed Engn TR-58140 Sivas Turkey;

    Cumhuriyet Univ Fac Pharm Dept Biochem TR-58140 Sivas Turkey;

    Inonu Univ Fac Pharm Dept Analyt Chem TR-44100 Malatya Turkey;

    Cumhuriyet Univ Fac Pharm Dept Analyt Chem TR-58140 Sivas Turkey;

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  • 正文语种 eng
  • 中图分类 化学;
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