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Electrochemical biosensor with graphene oxide nanoparticles and polypyrrole interface for the detection of bilirubin

机译：具有石墨烯氧化物纳米颗粒的电化学生物传感器和百吡咯接口检测胆红素

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摘要

A bilirubin biosensor was fabricated by immobilization of bilirubin oxidase (BOx) on a graphene oxide nanoparticle (GONP) decorated polypyrrole (Ppy) layer electrochemically deposited onto a fluorine doped tin oxide (FTO) glass plate. The enzyme electrode (BOx/GONP@Ppy/FTO), Ag/AgCl as the standard electrode and platinum as the auxiliary electrode were assembled using a potentiostat to develop an amperometric bilirubin biosensor. The characterization of the enzyme electrode was fulfilled using Raman spectroscopy, scanning electron microscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The biological sensor demonstrated a response at pH 7.5 and 30 degrees C in only 2 s which was optimum once polarized at +0.2 V vs. Ag/AgCl. The biosensor's limit of detection (LOD) and required limit of quantification (LOQ) was calculated as 0.1 nM (S/N = 3) and 2.6 nM, respectively. The electrocatalytic reaction illustrated a linear response over the bilirubin/substrate concentration in the range of 0.01 to 500 mu M. The half-life of the biosensor is 150 days, during which it could be reused 100 times after keeping the biosensor at 4 degrees C. The bilirubin levels measured in serum samples of both healthy and jaundice afflicted persons correlated well with the popular colorimetric method, the coefficient of determination (R-2) being 0.998.
机译：通过将胆红素氧化酶（盒）固定在石墨烯纳米颗粒（GONP）装饰的聚吡咯（PPY）层上以电化学沉积在氟掺杂氧化锡（FTO）玻璃板上的制造胆红素氧化酶（BOX）制备了胆红素生物传感器。使用恒温器组装酶电极（盒/ GONP @ PPY / FTO），AG / AGCL作为辅助电极作为辅助电极的铂，以开发电流致胆管素生物传感器。使用拉曼光谱，扫描电子显微镜，电化学阻抗光谱（EIS）和循环伏安法（CV）来满足酶电极的表征。生物传感器在pH 7.5和30摄氏度下表现出响应，仅2秒是最佳的一次偏振，在+ 0.2V与Ag / AgCl偏振。生物传感器的检测极限（LOD）和定量所需限制（LOQ）分别计算为0.1nm（S / N = 3）和2.6nm。电催化反应在0.01至500μm的范围内显示了胆红素/底物浓度的线性响应。生物传感器的半衰期为150天，在其在4摄氏度保持生物传感器后可以重复使用100次。 。在健康和黄疸的血清样品中测量的胆红素水平与普遍的比色法相关，测定系数（R-2）为0.998。

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  • 来源
    《RSC Advances》 |2016年第68期|共10页
  • 作者

    Chauhan N.; Rawal R.; Hooda V.; Jain U.;

  • 作者单位

    Amity Univ Amity Inst Nanotechnol Noida 201303 Uttar Pradesh India;

    Univ Delhi Dept Phys &

    Astrophys Delhi 110007 India;

    Maharshi Dayanand Univ Dept Bot Rohtak 124001 Haryana India;

    Amity Univ Amity Inst Nanotechnol Noida 201303 Uttar Pradesh India;

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  • 正文语种 eng
  • 中图分类 化学;
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