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A systematic investigation on the interaction of L-cysteine functionalised Mn3O4 nanoparticles with lysozyme

机译：L-半胱氨酸官能化Mn3O4纳米粒子与溶菌酶相互作用的系统研究

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摘要

Biological interactions of magnetic nanoparticles have attracted enormous interest because of their wide applications in medical diagnostics, imaging, and biosensors. Mn3O4 nanoparticles are potentially significant among magnetic nanoparticles and have not yet been investigated for their interactions with proteins. Here we report a systematic investigation of the interactions between hen egg white lysozyme and L-cysteine functionalised Mn3O4 nanoparticles using calorimetric and spectrophotometric techniques. The L-cysteine functionalised nanoparticles were synthesized by a simple chemical precipitation method and characterized by X-ray diffraction, FTIR, HRTEM, UV-Vis absorption and fluorescence spectroscopy. Mn3O4 nanoparticles are in the tetragonal phase with an average size of 10 nm. Thermodynamic parameters, the binding constant and the number of binding sites associated with the interactions were studied using Isothermal Titration Calorimetry (ITC). Quenching of lysozyme fluorescence is observed upon complex formation with Mn3O4 nanoparticle and the time resolved fluorescence measurements confirm static quenching. The Circular Dichroism (CD) spectra reveal minor alterations in the secondary structure of lysozyme in the complex indicating that Mn3O4 nanoparticles are potential candidates for therapeutic applications.
机译：由于其在医学诊断，成像和生物传感器中的广泛应用，磁性纳米粒子的生物相互作用引起了巨大的兴趣。 Mn3O4纳米颗粒在磁性纳米粒子中具有潜在的显着性，并且尚未研究其与蛋白质的相互作用。在这里，我们报告了使用量热量和分光光度法的母鸡蛋白溶菌酶和L-半胱氨酸官能化Mn3O4纳米颗粒之间的相互作用的系统研究。通过简单的化学沉淀方法合成L-半胱氨酸官能化纳米颗粒，其特征在于X射线衍射，FTIR，HRTEM，UV-Vis吸收和荧光光谱。 Mn3O4纳米颗粒处于四边形相位，平均尺寸为10nm。使用等温滴定量热法（ITC）研究了热力学参数，结合常数和与相互作用相关的结合位点的数量。在用Mn3O4纳米粒子复杂地层和分辨的荧光测量结果中观察到溶菌酶荧光的猝灭，确认静电猝灭。圆形二中间谱（CD）光谱揭示了溶菌酶的次结构中的微小改变，该络合物表明MN3O4纳米颗粒是治疗应用的潜在候选物。

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  • 来源
    《RSC Advances》 |2016年第107期|共11页
  • 作者

    Prasanth S.; Raj D. Rithesh; Thomas Riju K.; Vineeshkumar T. V.; Sudarsanakumar C.;

  • 作者单位

    Mahatma Gandhi Univ Kottayam Sch Pure &

    Appl Phys Kottayam 686560 Kerala India;

    Mahatma Gandhi Univ Kottayam Sch Pure &

    Appl Phys Kottayam 686560 Kerala India;

    Mahatma Gandhi Univ Kottayam Sch Pure &

    Appl Phys Kottayam 686560 Kerala India;

    Mahatma Gandhi Univ Kottayam Sch Pure &

    Appl Phys Kottayam 686560 Kerala India;

    Mahatma Gandhi Univ Kottayam Sch Pure &

    Appl Phys Kottayam 686560 Kerala India;

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  • 正文语种 eng
  • 中图分类 化学;
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