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首页> 外文期刊>RSC Advances >Interfacial processes that modulate the kinetics of lipase-mediated catalysis using porous silica host particles
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Interfacial processes that modulate the kinetics of lipase-mediated catalysis using porous silica host particles

机译：使用多孔二氧化硅宿主颗粒调节脂肪酶介导的催化作用的界面过程

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摘要

Surface immobilised lipases are important bioactive materials that have a wide range of applications in the biotechnology, chemical and pharmaceutical industries. However, the interfacial mechanism of action and the interplay between material characteristics and lipase activity are not well understood. A quartz crystal microbalance with dissipation (QCM-D) was used to elucidate interfacial processes between lipases and lipid films deposited on silica surfaces with varying wettabilities. Adsorption of triglycerides onto a hydrophilic support at multilayer coverage resulted in fast lipolysis kinetics, while adsorption onto a hydrophobic support hindered lipase activity and delayed lipolysis, characterised by changes in frequency and the release of free fatty acids from the QCM-D cell. In parallel, porous silica carriers with varying hydrophilicities/phobicities were used to confine lipid substrate molecules and manipulate lipase-mediated reactions, i.e. hydrolysis and esterification of triglycerides, as monitored using a pH-stat titrator. The surface chemistry of the carrier particles played a critical role on lipase action, whereby hydrophilic silica particles promoted catalysis of the hydrolysis reaction and hydrophobic particles promoted the reverse, esterification reaction. Physical observations of lipid film hydrolysis in combination with biomaterial design of lipid loaded porous silica particles provided advancements in understanding the mechanism of lipase action, which can be harnessed to tailor the delivery of poorly water-soluble molecules and improve the synthesis process of organic esters.
机译：表面固定化脂肪酶是生物技术，化学和制药行业中具有广泛应用的重要生物活性材料。然而，界面的作用机制和材料特征与脂肪酶活性之间的相互作用尚不清楚。具有耗散（QCM-D）的石英晶体微稳定用于阐明沉积在二氧化硅表面的脂肪酶和脂质膜之间的界面过程，其具有不同的湿润。甘油三酯的吸附在多层覆盖亲水性载体导致快速脂肪分解动力学，而吸附在疏水性载体受阻脂肪酶活性和延迟的脂肪分解，其特征在于，在频率和变化的游离脂肪酸从QCM-d细胞的释放。在平行的情况下，使用具有不同亲水性/植物的多孔二氧化硅载体来限制脂质基质分子并操纵脂肪酶介导的反应，即使用pH态滴定仪监测的甘油三酯的水解和酯化。载体颗粒的表面化学在脂肪酶作用上起关键作用，从而亲水性二氧化硅颗粒促进了水解反应的催化和疏水颗粒促进了反向酯化反应。脂质膜水解与脂质负载多孔二氧化硅颗粒的生物材料设计的物理观察提供了理解脂肪酶作用机理的进步，这可以利用来定制水溶性差的分子差，改善有机酯的合成过程。

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  • 来源
    《RSC Advances》 |2016年第49期|共12页
  • 作者

    Joyce Paul; Whitby Catherine P.; Prestidge Clive A.;

  • 作者单位

    Univ S Australia Sch Pharm &

    Med Sci City East Campus Adelaide SA 5001 Australia;

    Massey Univ Inst Fundamental Sci Private Bag 11222 Palmerston North 4442 New Zealand;

    Univ S Australia Sch Pharm &

    Med Sci City East Campus Adelaide SA 5001 Australia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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