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Hydropathy: the controlling factor behind the inhibition of A beta fibrillation by graphene oxide

机译：水疗法：石墨烯氧化物抑制β纤维化背后的控制因子

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摘要

Protein and peptide aggregation/fibrillation is reported to be responsible for several neurological disorders. Fibrillation of the amyloid beta-peptide fragment (25-35) which is a biologically active region of the full length peptide, has been observed to be significantly inhibited in presence of the two dimensional nanomaterial graphene oxide (GO). Fibrillation and inhibition of the A beta(25-35) peptide by GO has been performed at 37 degrees C at physiological pH (pH 7.4). The inhibition process is monitored by Thioflavin T fluorescence (ThT), circular dichroism spectroscopy, matrix assisted laser desorption/ionization mass spectrometry, dynamic light scattering experiments etc. The soluble fraction of the protein is quantified by the BCA assay. Microscopic techniques are used to study the morphology of the fibrils formed. GO is observed to inhibit the fibrillation even at very low concentrations and is amplified with increase in concentration of GO. ThT kinetic data fitted well with a sigmoidal curve and shows that GO is able to lengthen the lag phase of the fibrillation process. It appears that surface adsorption of protein on the nanomaterial prevents the monomers to come together. It is speculated that the presence of both polar and non-polar moieties in GO interact strongly with the hydrophobic and hydrophilic residues of the A beta(25-35) peptide monomer units, thus preventing further aggregation.
机译：据报道，蛋白质和肽聚集/纤维化是对几种神经疾病的原因。已经观察到是全长肽的生物活性区域的淀粉样蛋白β-肽片段（25-35）的原纤化，以在二维纳米材料石墨烯（GO）的存在下显着抑制。通过GOβ（25-35）肽的纤维化和抑制已经在37℃下在生理pH（pH7.4）下进行。通过硫蛋白T荧光（THT），圆形二色性光谱，基质辅助激光解吸/电离质谱，动态光散射实验等监测抑制作用。通过BCA测定量化蛋白质的可溶性部分。微观技术用于研究形成的原纤维的形态。即使在非常低的浓度下，观察到抑制原纤化，并且随着GO浓度的增加而扩增。动力学数据与丝状曲线合理，并表明可以延长原纤化过程的滞后阶段。似乎蛋白质在纳米材料上吸附的表面吸附可防止单体聚集在一起。推测，β（25-35）肽单体单元的疏水性和亲水残留，POL极性和非极性部分的存在强烈相互作用，从而防止进一步的聚集。

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  • 来源
    《RSC Advances》 |2016年第105期|共11页
  • 作者

    Bag Sudipta; Sett Ayantika; DasGupta Sunando; Dasgupta Swagata;

  • 作者单位

    Indian Inst Technol Kharagpur Dept Chem Kharagpur 721302 W Bengal India;

    Indian Inst Technol Kharagpur Dept Chem Engn Kharagpur 721302 W Bengal India;

    Indian Inst Technol Kharagpur Dept Chem Engn Kharagpur 721302 W Bengal India;

    Indian Inst Technol Kharagpur Dept Chem Kharagpur 721302 W Bengal India;

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  • 正文语种 eng
  • 中图分类 化学;
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