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Determination of riboflavin based on fluorescence quenching by graphene dispersions in polyethylene glycol

机译：聚乙二醇中石墨烯分散体的荧光猝灭测定核黄素

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摘要

The effect of graphene (G) dispersions in a biodegradable polymer, polyethylene glycol (PEG), on the fluorescence emission of vitamin B-2 (riboflavin) has been studied. The ultrasonication time and power used for the preparation of the dispersions have been optimized, and their quality has been evaluated by measuring the thickness of the G flakes and their distribution within the polymer by scanning electron microscopy (SEM), and by determining their defect content and degree of exfoliation by Raman spectroscopy. A quenching phenomenon of riboflavin fluorescence has been observed, attributed to pi-pi stacking interactions between the aromatic rings of the vitamin and G combined with PEG-riboflavin H-bonding interactions. The analysis of the fluorescence spectra confirms that, for mild ultrasonication conditions, the quenching becomes more effective on increasing G concentration, whilst for more intense conditions it is almost independent on the nanomaterial loading. Moreover, for a given G concentration, the fluorescence intensity increases slightly at low PEG contents, while it remains almost constant at high concentrations. The ratio data between the fluorescence intensity in the absence and in the presence of G fit to a second-order polynomial equation, suggesting a combined mechanism of static and dynamic quenching. The analytical characteristics of the fluorimetric method have been calculated for PEG aqueous solutions, for G dispersions in PEG and for the differences of intensities between them, and the best results were obtained in the presence of G. The proposed method was successfully applied to the analysis of riboflavin in multivitamin tablets, and the recoveries found in fortified samples were higher than 90%. The quenching phenomenon observed in this work could be employed for the development of new optical sensors for riboflavin determination.
机译：研究了石墨烯（G）分散在可生物降解的聚合物中的作用，聚乙二醇（PEG）对维生素B-2（核黄素）的荧光发射进行了研究。已经优化了用于制备分散体的超声波时间和功率，通过通过扫描电子显微镜（SEM）测量G薄片的厚度及其在聚合物内的分布，并通过确定它们的缺陷含量来评估它们的质量。拉曼光谱法和剥离程度。已经观察到核黄素荧光的猝灭现象，其归因于维生素的芳环与G与PEG-核黄素H键合相互作用之间的芳环与G之间的相互作用归因于PI-PI堆叠相互作用。荧光光谱的分析证实，对于温和的超声状况，猝灭变得更有效地对增加的G浓度，同时为了更强烈的条件，它几乎独立于纳米材料载荷。此外，对于给定的G浓度，荧光强度在低PEG含量下略微增加，而在高浓度下仍然几乎恒定。荧光强度与G适合于二阶多项式方程的荧光强度之间的比率数据，表明静态和动态淬火的组合机理。已经计算了PEG水溶液的荧光法的分析特性，用于PEG中的G分散，并且对于它们之间的强度差异，并且在G的存在下获得了最佳结果。该方法成功地应用于分析核黄素在多种维生素片中，并在强化样品中发现的回收率高于90％。在该工作中观察到的淬火现象可用于开发用于核黄素测定的新光学传感器。

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  • 来源
    《RSC Advances》 |2016年第24期|共14页
  • 作者

    Diez-Pascual Ana M.; Garcia-Garcia Diego; Paz San Andres Maria; Vera Soledad;

  • 作者单位

    Alcala Univ Fac Biol Environm Sci &

    Chem Analyt Chem Phys Chem &

    Chem Engn Dept E-28871 Madrid Spain;

    Alcala Univ Fac Biol Environm Sci &

    Chem Analyt Chem Phys Chem &

    Chem Engn Dept E-28871 Madrid Spain;

    Alcala Univ Fac Biol Environm Sci &

    Chem Analyt Chem Phys Chem &

    Chem Engn Dept E-28871 Madrid Spain;

    Alcala Univ Fac Biol Environm Sci &

    Chem Analyt Chem Phys Chem &

    Chem Engn Dept E-28871 Madrid Spain;

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