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首页> 外文期刊>RSC Advances >Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups
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Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups

机译：血管内皮生长因子的体积固定富含醌基富含兰酸官能化血浆聚合烯丙胺涂层的血管内皮生长因子

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摘要

Biomolecules with thiol or amine groups can easily be covalently immobilized onto a substrate equipped with quinone groups in a mild alkali buffer solution based on Schiff base or Michael addition reactions. In this study, we reported a simple two-step approach to creating a functional coating with abundant quinone groups for facile immobilization of vascular endothelial growth factor (VEGF) in mild phosphate buffered saline (PBS, pH 7.4). This approach initially involved the deposition of an amine-bearing plasma-polymerized allylamine (PPAam) coating. Tannic acid (TA) was subsequently used for introducing phenolic hydroxylic hydroxyl/quinone groups. The results of water contact angles (WCAs), Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy analysis (XPS) revealed the effective conjugation of TA to PPAam, as well as the immobilization of VEGF to TA-functionalized PPAam (TA-PPAam). The result of a quartz crystal microbalance with dissipation (QCM-D) showed that 158 +/- 13 ng cm(-2) of VEGF was successfully immobilized onto the TA-PPAam surface. TA-PPAam bound with VEGF significantly enhanced human umbilical vein endothelial cell (HUVEC) proliferation, indicating the good retention of the bioactivity of VEGF. The TA-PPAam functional coating provided a novel, facile strategy for the covalent immobilization of biomolecules, especially growth factors, under mild reaction conditions.
机译：具有硫醇或胺基团的生物分子可以容易地将基于Schiff碱或迈克尔添加反应的轻度碱缓冲溶液中配备有醌基团的基材上。在这项研究中，我们报道了一种简单的两步方法，以产生具有丰富的醌组的功能涂层，用于体育磷酸盐缓冲盐水（PBS，pH7.4）中的血管内皮生长因子（VEGF）的体内固定化。该方法最初涉及沉积含胺的等离子体聚合的烯丙胺（PPAAM）涂层。随后用于引入酚羟基羟基/醌基团的单宁酸（TA）。水接触角（WCAS），傅里叶变换红外光谱和X射线光电子能谱分析（XPS）揭示了TA至PPAAM的有效缀合，以及VEGF的固定到TA-官能化PPAAM（TA-PPAAM ）。具有耗散（QCM-D）的石英晶体微稳定的结果表明，将158 +/- 13ngcm（-2）的VEGF成功地固定在Ta-ppaam表面上。 TA-PPAAM与VEGF结合显着增强了人脐静脉内皮细胞（HUVEC）增殖，表明VEGF的生物活性良好。 Ta-PPAAM功能涂层提供了一种新颖的，适用于在轻度反应条件下的生物分子的共价固定，特别是生长因子的共价固定。

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  • 来源
    《RSC Advances》 |2016年第21期|共8页
  • 作者

    Xiong Kaiqin; Qi Pengkai; Yang Ying; Li Xiangyang; Qiu Hua; Li Xin; Shen Ru; Tu Qiufen; Yang Zhilu; Huang Nan;

  • 作者单位

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

    Southwest Jiaotong Univ Educ Minist Key Lab Adv Technol Mat Chengdu 610031 Peoples R China;

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