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首页> 外文期刊>RSC Advances >Determination of selective serotonin reuptake inhibitors in biological samples via magnetic stirring-assisted dispersive liquid-liquid microextraction followed by high performance liquid chromatography
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Determination of selective serotonin reuptake inhibitors in biological samples via magnetic stirring-assisted dispersive liquid-liquid microextraction followed by high performance liquid chromatography

机译：通过磁搅拌辅助分散液 - 液 - 微萃取测定生物样品中的选择性血清素再摄取抑制剂，其高效液相色谱

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摘要

This work is the first report of an efficient procedure for the simultaneous determination of five important selective serotonin reuptake inhibitors (SSRIs) in low concentration levels in biological fluids (urine and plasma samples), which is expedient, quick and of low cost. Despite the wide usage of citalopram, paroxetine, fluvoxamine, fluoxetine, and sertraline in the treatment of depression, and in spite of several advantages and developments of dispersive liquid-liquid microextraction (DLLME), there is not any report about the simultaneous preconcentration of these SSRIs using the DLLME technique. A developed mode of the DLLME technique, i.e., magnetic stirring-assisted dispersive liquid-liquid microextraction (MSA-DLLME), was employed and the parameters affecting the extraction process were optimized using a response surface methodology. The extraction method is based on the fast injection of a mixture of 1-octanol (extraction solvent) and methanol (disperser solvent) into the aqueous solution being stirred by a magnetic stirrer to form a cloudy ternary component solvent (aqueous solution, extracting solvent, disperser solvent) system. The potential variables affecting the extraction recovery such as the volume of the extraction and disperser solvents, pH of sample solution, salt addition, vortex time, and stirring rate were considered in the optimization process. A methodology according to a fractional factorial design (2(6-2)) was performed to choose the significant variables for optimization. The significant factors including the volume of the extraction solvent and the pH of the sample solution were then optimized using a central composite design (CCD). A quadratic model between dependent and independent variables was built and the optimum conditions were obtained. Under the optimum conditions, the proposed method was successfully applied for the determination of SSRIs in urine and plasma samples. Linearity (R-2 > 0.999) was obtained in the range of 2-1000 and 5-1000 ng mL(-1) for urine and 10-5000 and 25-5000 ng mL(-1) for the plasma samples. The extraction recoveries were between 75 and 97% and the preconcentration factors of SSRIs in urine and plasma samples were in the range of 14.03 to 17.94 and 2.82 to 3.57, respectively. Finally, the MSA-DLLME method was successfully applied for preconcentration and trace determination of SSRIs in biological matrices.
机译：这项工作是同时测定生物流体（尿液和血浆样品）低浓度水平的五个重要选择性血清素再摄取抑制剂（SSRIS）的高效步骤的第一个报告，这是有利的，快速和低成本。尽管大酞普兰，帕罗西汀，氟异恶胺，氟西汀和肉氨基素治疗抑郁症的含量，但尽管有几种优点和分散液 - 液体微萃取（DLLME）的发育，但没有关于这些同步前浓度的报告SSRIS使用DLLME技术。使用DLLME技术的开发模式，即磁搅拌辅助分散液 - 液微萃取（MSA-DILLME），并使用响应表面方法进行优化影响提取过程的参数。提取方法基于将1-辛醇（提取溶剂）和甲醇（分散剂溶剂）的混合物进入水溶液中的快速注射到通过磁力搅拌器搅拌的水溶液中以形成多浑浊的三元组分溶剂（水溶液，提取溶剂，分散器溶剂）系统。在优化过程中考虑了影响诸如萃取和分散器溶剂的体积，样品溶液，盐添加，涡旋时间和搅拌速率的诸如提取和分散器溶剂的体积的潜在变量。执行根据分数阶乘设计（2（6-2））的方法，以选择优化的显着变量。然后使用中央复合设计（CCD）优化包括提取溶剂的体积和样品溶液的pH值的重要因素。构建了依赖性和独立变量之间的二次模型，获得了最佳条件。在最佳条件下，已建议的方法成功地应用于测定尿液和血浆样品中的SSRIS。用于尿液和10-5000和25-5000ng ml（-1）的血浆样品，获得线性度（R-2> 0.999）的范围为2-1000和5-1000ng（-1）。提取回收率在75至97％之间，尿液中SSRI的前浓度和血浆样品的前浓度分别为14.03至17.94和2.82至3.57。最后，成功地应用了MSA-DLLME方法的生物基质中SSRI的前浓缩和痕量测定。

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  • 来源
    《RSC Advances》 |2016年第56期|共11页
  • 作者

    Golbabanezhadazizi A.; Ranjbari E.; Hadjmohammadi M. R.; Daneshinejad H.;

  • 作者单位

    Univ Mazandaran Fac Chem Dept Analyt Chem Babol Sar Iran;

    Univ Mazandaran Fac Chem Dept Analyt Chem Babol Sar Iran;

    Univ Mazandaran Fac Chem Dept Analyt Chem Babol Sar Iran;

    Shahrood Univ Technol Coll Chem POB 36155-316 Shahrood Iran;

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  • 正文语种 eng
  • 中图分类 化学;
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