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In vitro imaging of beta-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

机译：使用荧光立方体双连续液晶纳米粒子的β细胞体外成像

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摘要

To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic beta-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic beta-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 mu M in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for beta-cell imaging.
机译：为了评估基于Manoolein的立方体液晶结晶纳米颗粒（缔约置）作为糖尿病中的诊断工具的潜力，配制荧光齿状型，填充在脂质双层内的新合成的，有效的疏水染料。进行了Cryo-TEM，萨克斯和DLS以评估该配方的形态学和结构方面。大鼠胰腺β-细胞（INS-1E）容易占据委员会纳米颗粒，如通过体外内化试验所揭示的，流式细胞术分析证实荧光持续增长了24小时。通过对增殖的实时分析评价各种浓度以各种浓度的细胞毒性表明，相对于浓缩制剂的增殖曲线（在单烯醇含量方面为5μm）与未处理的培养物相当。 INS-1E细胞周期和凋亡支持此类结果。总之，该配方在纳米摩尔范围内具有染料含量，似乎可用于β细胞成像。

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  • 来源
    《RSC Advances》 |2016年第67期|共9页
  • 作者

    Miceli V.; Meli V.; Blanchard-Desce M.; Bsaibess T.; Pampalone M.; Conaldi P. G.; Caltagirone C.; Obiols-Rabasa M.; Schmidt J.; Talmon Y.; Casu A.; Murgia S.;

  • 作者单位

    IRCCS ISMETT Ist Mediterraneo iTrapianti &

    Terapie Ad Alta Spe Dept Lab Med &

    Adv Biotechnol Via Tricomi 5 I-90127 Palermo Italy;

    Univ Cagliari Dept Chem &

    Geol Sci CNBS CSGI Ss 554 Bivio Sestu I-09042 Monserrato Ca Italy;

    Univ Bordeaux Inst Mol Sci CNRS UMR 5255 F-33405 Talence France;

    Univ Bordeaux Inst Mol Sci CNRS UMR 5255 F-33405 Talence France;

    IRCCS ISMETT Ist Mediterraneo iTrapianti &

    Terapie Ad Alta Spe Dept Lab Med &

    Adv Biotechnol Via Tricomi 5 I-90127 Palermo Italy;

    IRCCS ISMETT Ist Mediterraneo iTrapianti &

    Terapie Ad Alta Spe Dept Lab Med &

    Adv Biotechnol Via Tricomi 5 I-90127 Palermo Italy;

    Univ Cagliari Dept Chem &

    Geol Sci CNBS CSGI Ss 554 Bivio Sestu I-09042 Monserrato Ca Italy;

    Dept Chem Div Phys Chem Nat Vetarvagen 14 SE-22362 Lund Sweden;

    Technion Israel Inst Technol Dept Chem Engn IL-3200003 Haifa Israel;

    Technion Israel Inst Technol Dept Chem Engn IL-3200003 Haifa Israel;

    IRCCS ISMETT Dept Study &

    Treatment Abdominal Dis &

    Transplant Diabet Serv Ist Mediterraneo iTrapianti &

    Terapie Ad Alta Spe Via Tricomi 5 I-90127 Palermo Italy;

    Univ Cagliari Dept Chem &

    Geol Sci CNBS CSGI Ss 554 Bivio Sestu I-09042 Monserrato Ca Italy;

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