Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization

Zhou Liya; Tang Wei; Jiang Yanjun; Ma Li; He Ying; Gao Jing

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Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization

机译：猪皮过氧化物酶和葡萄糖氧化酶的磁性组合交联酶聚集体：染料脱色的有效生物催化剂

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In this study, the magnetic combined cross-linked enzyme aggregates (combi-CLEAs) of glucose oxidase (GOD) and horseradish peroxidase (HRP) were designed and prepared successfully. The magnetic combi-CLEAs were fabricated by co-precipitation and cross-linking for the immobilization of GOD and HRP onto amino-functionalized Fe3O4 particles. Ethanol and glutaraldehyde were used as precipitant and cross-linker, respectively. In order to evaluate the catalytic performance, the magnetic combi-CLEAs were applied in the removal of direct black-38 (DB38). The results indicated that the magnetic combi-CLEAs exhibited high removal efficiency for DB38 at pH 6.0 and 40 degrees C. Under optimal conditions, the highest removal efficiency of DB38 was 92.28%, which was higher than that of free enzymes (46.82%). After 8 cycles of reuse, the DB38 removal efficiency could still reach 55.53%, indicating that the magnetic combi-CLEAs had good reusability. Due to the easy separation and high reusability, this study shows that magnetic combi-CLEAs may potentially be an environmentally friendly and cost effective biocatalyst for dye removal and water treatments.

机译：在该研究中，设计并成功地设计并制备了葡萄糖氧化酶（GOD）和辣根过氧化物酶（HRP）的磁性组合交联酶聚集体（COMBI-CLE）。通过共沉淀和交联以将上帝和HRP固定到氨基官能化Fe3O4颗粒上的交联磁性组合物。乙醇和戊二醛分别用作沉淀剂和交联剂。为了评估催化性能，磁性组合物包在去除直接黑色-38（DB38）中。结果表明，在最佳条件下，在pH6.0和40℃下表现出DB38的高除去效率。DB38的最高去除效率为92.28％，高于游离酶（46.82％）。在8个循环重用后，DB38去除效率仍然可以达到55.53％，表明磁性组合小组具有良好的可重用性。由于易于分离和高可重复使用性，该研究表明，磁性组合物植物可能是一种环保和成本效益的生物催化剂，用于染料去除和水处理。

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《RSC Advances》 |2016年第93期|共8页
作者
Zhou Liya; Tang Wei; Jiang Yanjun; Ma Li; He Ying; Gao Jing;
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作者单位

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

Hebei Univ Technol Sch Chem Engn &

Technol Tianjin 300130 Peoples R China;

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正文语种 eng
中图分类化学;
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1. Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization [J] . Zhou Liya, Tang Wei, Jiang Yanjun, RSC Advances . 2016,第93期

机译：猪皮过氧化物酶和葡萄糖氧化酶的磁性组合交联酶聚集体：染料脱色的有效生物催化剂
2. Characterization of combined cross-linked enzyme aggregates from laccase, versatile peroxidase and glucose oxidase, and their utilization for the elimination of Pharmaceuticals [J] . Imad E. Touahar, Lounes Haroune, Sidy Ba, Science of the total environment . 2014,第maya15期

机译：漆酶，通用过氧化物酶和葡萄糖氧化酶组合的交联酶聚集体的表征及其在消除药物中的用途
3. Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: Production, partial characterization and application for the elimination of endocrine disruptors [J] . Taboada-Puig R., Junghanns C., Demarche P., Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2011,第11期

机译：多种过氧化物酶和葡萄糖氧化酶的组合交联酶聚集体：消除内分泌干扰物的生产，部分表征和应用
4. Bienzymatic glucose biosensor based on co-immobilization of glucose oxidase and horseradish peroxidase on gold nanoparticles-mesoporous silica matrix [C] . 2008 IEEE纳米电子学国际会议暨上海纳光电论坛(2nd IEEE International Nanoelectronics Conference Shanghai Nanophotonics and Electronics Forum)论文集 . 2008

机译：基于葡萄糖氧化酶和辣根过氧化物酶共同固定在金纳米粒子-中孔二氧化硅基质上的双酶葡萄糖生物传感器
5. Part I. Magnetic circular dichroism studies of cytochrome c peroxidase from yeast: Investigations of the active site heme coordination sphere through comparison with horseradish peroxidase and myoglobin. Part II. Examination of the heme active site coordination structure of the cavity mutants of myoglobin (H93G), cytochrome c peroxidase (H175G), and heme oxygenase (H25A) and structural investigation of thiolate-ligated cytochrome c peroxidase in the H17C/D235L double mutant. [D] . Pond, Alycen Easterling. 1999

机译：第一部分。酵母中细胞色素C过氧化物酶的磁性圆二色性研究：通过与辣根过氧化物酶和肌红蛋白的比较，研究了活性部位血红素配位域。第二部分检查肌红蛋白（H93G），细胞色素c过氧化物酶（H175G）和血红素加氧酶（H25A）的空腔突变体的血红素活性位点配位结构，以及H17C / D235L双突变体中硫醇盐连接的细胞色素c过氧化物酶的结构研究。
6. Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis [O] . Álvaro Cruz-Izquierdo, Enrique A. Picó, Carmen López, -1

机译：南极假丝酵母脂肪酶的磁性交联酶聚集体（mCLEAs）：一种高效稳定的生物柴油合成生物催化剂。
7. Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica lipase:An efficient and stable biocatalyst for biodiesel synthesis [O] . Cruz-Izquierdo, Álvaro, Picó, Enrique A., López, Carmen, 2014

机译：南极假丝酵母脂肪酶的磁性交联酶聚集体（mCLEAs）：一种高效，稳定的生物柴油合成生物催化剂
8. Acquisition of Heat Stable Enzymes from Thermophilic Microorganisms: Peroxidases,Ureases, and Glucose Oxidases [R] . Combie, J. D., Runnion, K. N., Williamson, M. L. 1992

机译：从嗜热微生物中获取热稳定酶：过氧化物酶，脲酶和葡萄糖氧化酶

Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization

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