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首页> 外文期刊>RSC Advances >Fabrication and characterization of soft macroporous Jeffamine cryogels as potential materials for tissue applications
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Fabrication and characterization of soft macroporous Jeffamine cryogels as potential materials for tissue applications

机译：软巨孔jeffamine Cryogels作为组织应用的潜在材料的制造与表征

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摘要

Macroporous three-dimensional (3D) scaffolds have been proven to function as structural substrates and as apertures for cell growth and proliferation thereby ensuring tissue regeneration. Such scaffolds demonstrate high pore interconnectivity and functionality and as a result permit cell adherence and growth while maintaining mechanical strength, biodegradability and biocompatibility without causing any immune response. In this study, macroporous cryogels were synthesized from difunctional Jeffamine (600, 900 and 2000 g mol(-1)) and glutaraldehyde in the presence of a trifunctional Jeffamine (403 g mol(-1)) as a crosslinker via a freeze-drying technique. The fabricated cryogels were subsequently reduced by reductive amination to create more active functional groups which increase biological interaction. The functional groups, reductive efficacy, pore size/surface topology and thermal stability were analysed using FT-IR and C-13-NMR, SEM and TGA, respectively. Visco-elastic behaviour of the cryogels was investigated by unconfined compression test which depicted good elastic modulus and mechanical strength. Considerable swelling ratios were obtained alongside in vitro degradation analysis performed for 10 days at pH 7.4. In addition, in vitro biocompatibility was evaluated via MTT assay for 24 h at 37 degrees C using Human Umbilical Vein Endothelial Cells (HUVEC) for cell growth and cytotoxicity evaluation on the non-reduced and reduced Jeffamine cryogels.
机译：已经证明了大孔三维（3D）支架以用作结构基质和用于细胞生长和增殖的孔，从而确保组织再生。这种支架表明了高孔隙互连性和功能，并且结果允许细胞粘附和生长，同时保持机械强度，生物降解性和生物相容性，而不会导致任何免疫应答。在该研究中，通过双官能jeffamine（600,900和2000g mol（-1））和在三官能jeffamine（403g mol（-1））存在下作为交联剂，通过冷冻干燥来合成大孔冷冻蛋白和戊二醛技术。通过还原胺化随后减少制造的冷冻蛋白以产生增加生物相互作用的更活跃的官能团。使用FT-IR和C-13-NMR，SEM和TGA分别分析官能团，还原效率，孔径/表面拓扑和热稳定性。通过非整合的压缩试验研究了冷冻胶质的粘弹性行为，其描绘了良好的弹性模量和机械强度。在pH7.4下在体外降​​解分析中，在体外降解分析中获得了相当大的溶胀比。此外，使用人的脐静脉内皮细胞（Huvec）在37℃下通过MTT测定法评估体外的生物相容性，用于对细胞生长和对非减少和减少的Jeffamine Cryogels进行细胞生长和细胞毒性评估。

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  • 来源
    《RSC Advances》 |2016年第113期|共10页
  • 作者

    Erdem Ahmet; Ngwabebhoh Fahanwi Asabuwa; Yildiz Ufuk;

  • 作者单位

    Kocaeli Univ Dept Chem Umuttepe Campus TR-41380 Kocaeli Turkey;

    Kocaeli Univ Dept Chem Umuttepe Campus TR-41380 Kocaeli Turkey;

    Kocaeli Univ Dept Chem Umuttepe Campus TR-41380 Kocaeli Turkey;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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