Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene

Hong Shanni; Sun Na; Liu Min; Wang Jine; Pei Renjun

首页> 外文期刊>RSC Advances >Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene

【24h】

Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene

机译：为沉积半乳糖蛋白-1基因构建Aptamer-反义寡核苷酸的嵌合体

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Galectin-1 is closely related with immune systems, and its overexpression may cause tumor metastasis. Owing to the better stability of antisense oligonucleotides than siRNA, we constructed a chimera of aptamer-antisense oligonucleotide (Apt-AS) for silencing the galectin-1 gene specifically. For studying the endocytosis pathways of Apt-AS by aptamer targeted delivery, we marked the 3' terminus of Apt-AS with Alexa Flour 488 (Apt-AS488) so as to trace the pathways through the confocal microscope. Furthermore, Apt-AS was used to silence the expression of galectin-1. The results showed Apt-AS entered cells by a caveolae mediated endocytosis pathway, and the aptamer-antisense chimeras did not impair the antisense gene silencing efficiency. Moreover Apt-AS could improve the cellular uptake and selectively entered cells.

机译：Galectin-1与免疫系统密切相关，其过表达可能导致肿瘤转移。由于较好的反义寡核苷酸的稳定性而不是siRNA，我们构成了一种特异性的沉积抗敏 - 反义寡核苷酸（apt-as）的嵌合体，用于具体沉默加塞霉素-1基因。用于研究Apt的内吞作用 - 通过适体靶向递送，我们标记为Apt-Apt-asα面粉488（apt-as488），以便通过共聚焦显微镜追踪途径。此外，Apt-as用于沉默Galectin-1的表达。结果表明Caveolae介导的内吞作用途径的进入细胞，并且Aptamer-反义嵌合乳渣不会损害反义基因沉默效率。此外，APT-AS可以改善细胞吸收和选择性进入细胞。

著录项

来源
《RSC Advances》 |2016年第113期|共6页
作者
Hong Shanni; Sun Na; Liu Min; Wang Jine; Pei Renjun;
展开▼
作者单位

Univ Sci &

Technol China Sch Nano Technol &

Nano Bion Hefei 230026 Peoples R China;

Chinese Acad Sci Suzhou Inst Nanotech &

Nanobion Suzhou 215123 Peoples R China;

Chinese Acad Sci Suzhou Inst Nanotech &

Nanobion Suzhou 215123 Peoples R China;

Chinese Acad Sci Suzhou Inst Nanotech &

Nanobion Suzhou 215123 Peoples R China;

Chinese Acad Sci Suzhou Inst Nanotech &

Nanobion Suzhou 215123 Peoples R China;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类化学;
关键词

相似文献

外文文献
中文文献
专利

1. Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene [J] . Hong Shanni, Sun Na, Liu Min, RSC Advances . 2016,第113期

机译：为沉积半乳糖蛋白-1基因构建Aptamer-反义寡核苷酸的嵌合体
2. Next-Generation Peptide Nucleic Acid Chimeras Exhibit High Affinity and Potent Gene Silencing [J] . Debacker Alexandre J., Sharma Vivek K., Krishnamurthy Pranathi Meda, Biochemistry . 2019,第6期

机译：下一代肽核酸嵌合体表现出高亲和力和有效的基因沉默
3. Galectin-1 gene silencing inhibits the activation and proliferation but induces the apoptosis of hepatic stellate cells from mice with liver fibrosis [J] . JiangZhi?Jun, ShenQing?Hua, ChenHai?Yong, International journal of molecular medicine . 2019,第1期

机译：Galectin-1基因沉默抑制激活和增殖，但诱导肝纤维化小鼠肝星状细胞凋亡
4. Synthesis of and Gene Silencing by Oligonucleotide-peptide Conjugates [C] . Masayuki Fujii Japanese peptide symposium . 2011

机译：寡核苷酸肽缀合物的合成和基因沉默
5. Chemistry for gene silencing: 4'-modified and 2'-fluorinated nucleosides and oligonucleotides. [D] . Watts, Jonathan K. 2008

机译：基因沉默化学：4'-修饰的和2'-氟化的核苷和寡核苷酸。
6. Galectin-1 gene silencing inhibits the activation and proliferation but induces the apoptosis of hepatic stellate cells from mice with liver fibrosis [O] . Zhi-Jun Jiang, Qing-Hua Shen, Hai-Yong Chen, -1

机译：Galectin-1基因沉默抑制肝纤维化小鼠肝星状细胞的活化和增殖但诱导其凋亡
7. Antisense inhibition of gene expression in cells by oligonucleotides incorporating locked nucleic acids: effect of mRNA target sequence and chimera design [O] . Braasch, Dwaine A., Liu, Yinghui, Corey, David R. 2002

机译：包含锁定核酸的寡核苷酸对细胞中基因表达的反义抑制：mRNA靶序列和嵌合体设计的影响

Building a chimera of aptamer-antisense oligonucleotide for silencing galectin-1 gene

摘要

著录项

相似文献

相关主题

期刊订阅