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首页> 外文期刊>RSC Advances >Cationic bovine serum albumin (CBA) conjugated poly lactic-co-glycolic acid (PLGA) nanoparticles for extended delivery of methotrexate into brain tumors
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Cationic bovine serum albumin (CBA) conjugated poly lactic-co-glycolic acid (PLGA) nanoparticles for extended delivery of methotrexate into brain tumors

机译：阳离子牛血清白蛋白（CBA）缀合的聚乳酸共甘油酸（PLGA）纳米颗粒，用于将甲氨蝶呤延伸到脑肿瘤中

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摘要

Current strategies for the treatment of brain tumors have been hindered primarily by the presence of the highly lipophilic, insurmountable blood-brain barrier (BBB). The purpose of the current research is to investigate the efficiency of the engineered biocompatible polymeric nanoparticles (NPs) as drug delivery vehicles to bypass the BBB and enhance the biopharmaceutical attributes of anti-metabolite methotrexate (MTX) encapsulated NPs. The NPs were prepared by the solvent diffusion method, using cationic bovine serum albumin (CBA), and were characterized for physicochemical parameters, such as particle size, polydispersity index, and zeta-potential, while the surface modification was confirmed by FTIR, and NMR spectroscopy. The developed NPs exhibited the zestful relocation of FITC tagged NPs across the BBB in albino rats. Further, hemolytic studies confirmed them to be non-toxic and biocompatible, as compared to free MTX. The in vitro cytotoxicity assay of our engineered NPs on HNGC1 tumor cells proved the superior uptake in tumor cells, and elicited a potent cytotoxic effect, compared to plain NPs and the free MTX solution. The outcomes of the study evidently indicate the prospect of CBA conjugated poly (D,L-lactide-co-glycolide) (PLGA) NPs loaded with MTX in a brain cancer bomber, with the amplified capability to circumvent the BBB.
机译：目前用于治疗脑肿瘤的策略主要是通过高脂潜水，难以克服的血脑屏障（BBB）的存在来阻碍。目前研究的目的是研究工程化生物相容性聚合物纳米颗粒（NPS）作为药物递送载体的效率，以绕过BBB并增强抗代谢物甲氨蝶呤（MTX）包封的NPS的生物制药属性。使用阳离子牛血清白蛋白（CBA）的溶剂扩散方法制备NPS，其特征在于物理化学参数，例如粒度，多分散指数和Zeta-电位，而FTIR和NMR确认表面改性光谱学。开发的NPS在白化大鼠中，在BBB上表现出FITC标记的NPS的热情重新定位。此外，与游离MTX相比，溶血研究证实它们是无毒和生物相容的。我们在HNGC1肿瘤细胞上进行工程化NPS的体外细胞毒性测定证明了肿瘤细胞的高级摄取，并引发了与普通NPS和游离MTX溶液相比的有效的细胞毒性作用。研究结果明显表示CBA缀合的聚（D，L-丙交酯 - 共糖苷）（PLGA）NPS的前景在脑癌轰炸机中加载MTX，具有扩增的能力来规避BBB。

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  • 来源
    《RSC Advances》 |2016年第92期|共11页
  • 作者

    Kesharwani Prashant; Jain Ashay; Jain Atul; Jain Amit K.; Garg Neeraj Kumar; Tekade Rakesh Kumar; Singh Thakur Raghu Raj; Iyer Arun K.;

  • 作者单位

    Int Med Univ Sch Pharm Dept Pharmaceut Technol Kuala Lumpur 57000 Malaysia;

    Dr Hari Singh Gour Vishwavidyalaya Dept Pharmaceut Sci Sagar 470003 MP India;

    Dr Hari Singh Gour Vishwavidyalaya Dept Pharmaceut Sci Sagar 470003 MP India;

    Dr Hari Singh Gour Vishwavidyalaya Dept Pharmaceut Sci Sagar 470003 MP India;

    Dr Hari Singh Gour Vishwavidyalaya Dept Pharmaceut Sci Sagar 470003 MP India;

    NIPER Sarkhej Gandhinagar Highway Ahmadabad 380054 Gujarat India;

    Queens Univ Belfast Ctr Med Biol Sch Pharm 97 Lisburn Rd Belfast BT9 7BL Antrim North Ireland;

    Wayne State Univ Eugene Applebaum Coll Pharm &

    Hlth Sci Dept Pharmaceut Sci Use Inspired Biomat &

    Integrated Nano Delivery U 259 Mack Ave Detroit MI 48201 USA;

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  • 正文语种 eng
  • 中图分类 化学;
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