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Super-resolution imaging of STAT3 cellular clustering during nuclear transport

机译：核运输期间Stat3细胞聚类的超级分辨率成像

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摘要

Signal transducer and activator of transcription 3 (STAT3) mediates signals from the plasma membrane to the nucleus, and participates in gene expression in response to cytokines and growth factors. STAT3 translocation to the cell nucleus and subsequent recycling into the cytoplasm is the critical step for multiple functions. However, the distribution and molecular composition of STAT3 during the nuclear transport upon activation by different stimuli remains largely elusive. By direct stochastic optical reconstruction microscopy (dSTORM), we visualized the nucleocytoplasmic distribution of STAT3 in HeLa cells following different stimuli. Our work reveals that STAT3 is preclustered in the cytoplasm before activation, and that interleukin-6 promotes the formation of more and larger clusters. Further analysis indicates that STAT3 clusters vary with the nuclear translocation of STAT3. Moreover, dual-color dSTORM imaging shows the colocalization of STAT3 and STAT1 clusters, and suggests that the ratios of their colocalization are associated with STAT functions.
机译：转录3（STAT3）的信号传感器和活化剂介导从血浆膜到核的信号，并参与基因表达以响应细胞因子和生长因子。 STAT3对细胞核的易位和随后再循环到细胞质中是多种功能的关键步骤。然而，不同刺激激活后核转运期间Stat3的分布和分子组成仍然很大程度上是难以捉摸的。通过直接随机光学重建显微镜（Dstorm），在不同刺激后，我们在HeLa细胞中显示了STAT3的核细胞质分布。我们的作品揭示了STAT3在激活前在细胞质中印发，并且白细胞介素-6促进了较大簇的形成。进一步的分析表明Stat3集群随着Stat3的核易位而变化。此外，双色Dstorm成像显示STAT3和Stat1集群的分致化，并表明其分层化的比率与统计功能相关联。

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  • 来源
    《RSC Advances》 |2016年第59期|共11页
  • 作者

    Gao Jing; Wang Feng; Chen Junling; Wang Jianzhong; Cai Mingjun; Xu Haijiao; Jiang Junguang; Wang Hongda;

  • 作者单位

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

    Jilin Univ Bethune Hosp Acad Translat Med 1 Inst Immunol Changchun 130023 Peoples R China;

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

    NE Normal Univ Sch Comp Sci &

    Informat Technol Changchun Peoples R China;

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

    Chinese Acad Sci Changchun Inst Appl Chem State Key Lab Electroanalyt Chem Changchun 130022 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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