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Synthesis, characterization and stability of BSA-encapsulated microbubbles

机译：BSA封装微泡的合成，表征和稳定性

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摘要

In this work, we present an account of experimental studies performed for the synthesis, shelf stability and in vitro stability of microbubbles made from perfluorobutane (PFB) gas and coated in a shell of Bovine Serum Albumin (BSA). These microbubbles were produced by probe sonication method using formulations containing BSA, caprylic acid (CA) and N-acetyl-DL-tryptophan (Tryp) in different combinations. The freshly prepared polydisperse (0.5-20 mu m) microbubble samples were then size isolated by centrifugal differentiation to produce microbubble suspensions with a narrow size range of 3 to 5 mu m. Among all the different combinations of BSA, CA and Tryp used, the formulation containing BSA and Tryp yields microbubbles with a maximum shelf life (of similar to 8 months). This stability was observed when microbubbles were stored in an aqueous solution (80% v/v) consisting of the original solution [which contains BSA and Tryp dissolved in phosphate buffer saline (PBS)] used to make the microbubbles, 1,2-propane-diol (10% v/v) and glycerin (10% v/v). Fluorescence and Circular Dichroism (CD) spectroscopic analyses were carried out to study the effect of additives such as CA and Tryp, and heating and sonication on the secondary and tertiary structure of BSA. It was found that the use of Tryp in the formulation favors greater unfolding of BSA as compared to CA. This in addition to a lower diffusivity of PFB, and lower solubility of BSA in the solution containing Tryp leads to a relatively stable microbubble suspension. Microbubbles produced using different BSA formulations were mixed with PBS at 37 degrees C to check in vitro stability of the microbubbles. It was observed that microbubbles produced from BSA and Tryp persisted longer than other microbubbles. In addition to this, in vitro ultrasonography (USG) carried out using freshly prepared microbubbles and microbubbles stored for around 4 months also yielded promising results.
机译：在这项工作中，我们提出了对由全氟丁烷（PFB）气体制成的微泡的合成，货架稳定性和微泡的体外稳定性进行的实验研究的陈述陈述，并涂覆在牛血清白蛋白（BSA）的壳中。这些微泡由探针超声处理方法使用含有BSA，辣椒酸（CA）和N-乙酰基-DL-色氨酸（Tryp）的制剂以不同组合的制剂生产。然后，通过离心分化分离出新鲜制备的多分散（0.5-20μm）微胶石样品，以产生微泡悬浮液，窄尺寸范围为3至5μm。在使用的BSA，Ca和Tryp的所有不同组合中，含有BSA和TROP的制剂产生微泡，其具有最大保质期（类似于8个月）。当微泡储存在水溶液（80％v / v）中的原始溶液中，观察到这种稳定性，所述原始溶液[含有溶解在磷酸盐缓冲盐水（PBS）中的BSA和胰蛋白酶）的原始溶液中，用于制造微泡，1,2-丙烷 - 二醇（10％v / v）和甘油（10％v / v）。进行荧光和圆形二色性（CD）光谱分析，以研究添加剂如Ca和Tryp等添加剂，以及对BSA的二次和三级结构进行加热和超声。有人发现，与CA相比，在制剂中使用Tryp的使用更加展开BSA。这除了PFB的较低扩散性之外，BSA在含有ktp的溶液中的低溶解度也导致相对稳定的微泡悬浮液。使用不同的BSA制剂产生的微泡与37摄氏度的PBS混合，检查微泡的体外稳定性。观察到从BS​​A和Tryp产生的微泡持续时间比其他微泡长。除此之外，使用新制备的微泡和储存的微泡来进行体外超声检查（USG），还产生了有前途的结果。

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  • 来源
    《RSC Advances》 |2016年第18期|共11页
  • 作者

    Upadhyay Awaneesh; Dalvi Sameer V.;

  • 作者单位

    Indian Inst Technol Gandhinagar Chem Engn Chandkheda 382424 India;

    Indian Inst Technol Gandhinagar Chem Engn Chandkheda 382424 India;

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  • 正文语种 eng
  • 中图分类 化学;
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