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An approach towards continuous production of silver nanoparticles using Bacillus thuringiensis

机译：使用芽孢杆菌连续生产银纳米粒子的方法

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摘要

Applying the principles of green chemistry for the synthesis of nanoparticles (NPs) is an emerging field in the current era. Continuous production of NPs, i. e. simultaneous bacterial growth and NP production, if possible, would be more effective for different NP mediated applications. Hence, our approach here is to optimize the method to produce and extract silver nanoparticles (AgNPs) during growth of the bacteria Bacillus thuringiensis. The fabricated AgNPs were obtained when the bacteria were grown at a minimum inhibitory concentration of AgNO3 in culture medium. The microorganism produced elemental silver NPs with particular surface physico-chemical properties pertaining to the cellular moieties acting as a capping agent. Fabrication of the NPs was confirmed using UV-Vis absorbance spectra, attenuated total reflection Fourier transform infrared spectra, zeta analysis, and field emission scanning electron micrographs. The UV-Vis and IR studies together indicated the presence of proteins on the NP surface. The fabricated NPs were further purified using size exclusion chromatography (SEC), and the presence of the NPs in different elutions was further confirmed using UV-Vis, IR spectroscopes, and TEM. The yield, upon purification, was 98.75 mg of AgNPs from 500 mL of the culture, which is a relatively good yield. Additionally, the purified AgNPs were found to have a relatively stronger antibacterial activity against Escherichia coli than commercially available AgNPs. The work shows that a microorganism with resistance to significant concentrations of metal ions can be used for continuous production of metal NPs for industrial as well as biological applications.
机译：应用绿色化学原理在合成纳米粒子（NPS）是当前时代的新兴领域。连续生产NPS，我。 e。同时细菌生长和NP生产，如果可能的话，对不同的NP介导的应用更有效。因此，我们这里的方法是优化在细菌芽孢杆菌生长期间生产和提取银纳米颗粒（AgNP）的方法。当细菌在培养基中的末端抑制浓度的最低抑制浓度生长时，获得制造的agnps。微生物产生了元素的银NP，具有与作为封端剂的细胞部分有关的特定表面物理化学性质。使用UV-Vis吸光度谱进行确认NPS的制备，减弱全反射傅里叶变换红外光谱，Zeta分析和场发射扫描电子显微照片。 UV-Vis和IR研究将在一起表明存在NP表面上的蛋白质。使用尺寸排阻色谱法（SEC）进一步纯化制造的NPS，并使用UV-Vis，IR光谱和TEM进一步证实了NPS在不同的洗脱中的存在。纯化的产率为500ml培养物的98.75mg AgNP，其产率相对较好。另外，发现纯化的AgNPS具有与来自商业上可获得的agnps具有相对较强的抗菌活性。该工作表明，具有对显着浓度金属离子的耐药性的微生物可用于连续生产工业和生物应用的金属NPS。

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  • 来源
    《RSC Advances》 |2016年第10期|共11页
  • 作者

    Nayak Parth Sarthi; Arakha Manoranjan; Kumar Ajeet; Asthana Shreyasi; Mallick Bairagi C.; Jha Suman;

  • 作者单位

    Natl Inst Technol Rourkela Dept Life Sci Odisha 769008 India;

    Natl Inst Technol Rourkela Dept Life Sci Odisha 769008 India;

    Natl Inst Technol Rourkela Dept Life Sci Odisha 769008 India;

    Natl Inst Technol Rourkela Dept Life Sci Odisha 769008 India;

    Ravenshaw Univ Dept Chem Cuttack 753003 Odisha India;

    Natl Inst Technol Rourkela Dept Life Sci Odisha 769008 India;

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  • 正文语种 eng
  • 中图分类 化学;
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